ZFIN ID: ZDB-PUB-111117-27
Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-Hydroxysteroid dehydrogenase type 2
Tokarz, J., Mindnich, R., Norton, W., Möller, G., Hrabé de Angelis, M., and Adamski, J.
Date: 2012
Source: Molecular and Cellular Endocrinology   349(2): 202-13 (Journal)
Registered Authors: Norton, Will
Keywords: cortisol, 20beta-HSD, zebrafish, metabolism, stress
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Cortisone/metabolism
  • Cortisone Reductase/genetics
  • Cortisone Reductase/metabolism*
  • Endoplasmic Reticulum/metabolism
  • Female
  • Fish Proteins/genetics
  • Fish Proteins/metabolism*
  • Gene Expression Regulation, Developmental
  • Genetic Association Studies
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Hydrocortisone/metabolism*
  • Isoenzymes/genetics
  • Isoenzymes/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Transfection
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
PubMed: 22061621 Full text @ Mol. Cell. Endocrinol.
Hydroxysteroid dehydrogenases (HSDs) are involved in metabolism and pre-receptor regulation of steroid hormones. While 17beta-HSDs and 11beta-HSDs are extensively studied in mammals, only few orthologs are characterized in fish. We discovered a novel zebrafish HSD candidate closely related to 17beta-HSD types 3 and 12, which has orthologs in other species. The enzyme catalyzes the conversion of cortisone to 20beta-hydroxycortisone identified by LC-MS/MS. We named the new enzyme 20beta-HSD type 2. All 20beta-HSD type 2 orthologs localize in the endoplasmic reticulum. Zebrafish 20beta-HSD type 2 is expressed during embryonic development showing the same expression pattern as 11beta-HSD type 2 known to oxidize cortisol to cortisone. In adult tissues 20beta-HSD type 2 shows a ubiquitous expression pattern with some minor sex-specific differences. In contrast to other enzymes metabolizing C21-steroids and being mostly involved in reproduction we propose that novel type 2 20beta-HSDs in teleost fish are important enzymes in cortisol catabolism.