ZFIN ID: ZDB-PUB-111114-4
Apical migration of nuclei during G2 is a prerequisite for all nuclear motion in zebrafish neuroepithelia
Leung, L., Klopper, A.V., Grill, S.W., Harris, W.A., and Norden, C.
Date: 2011
Source: Development (Cambridge, England)   138(22): 5003-5013 (Journal)
Registered Authors: Harris, William A., Norden, Caren
Keywords: cell cycle, interkinetic nuclear migration, pseudostratified epithelia, quantitative analysis, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Cell Nucleus/genetics
  • Cell Nucleus/metabolism
  • Cell Nucleus/physiology*
  • Cell Polarity/genetics
  • Cell Polarity/physiology
  • Cell Proliferation
  • Embryo, Nonmammalian
  • G2 Phase/genetics
  • G2 Phase/physiology*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Movement/physiology
  • Neuroepithelial Cells/metabolism
  • Neuroepithelial Cells/physiology
  • Neuroepithelial Cells/ultrastructure*
  • Proliferating Cell Nuclear Antigen/genetics
  • Proliferating Cell Nuclear Antigen/metabolism
  • Retina/cytology
  • Retina/embryology
  • Retina/metabolism
  • Retina/physiology
  • Rhombencephalon/cytology
  • Rhombencephalon/embryology
  • Rhombencephalon/metabolism
  • Rhombencephalon/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish/physiology
PubMed: 22028032 Full text @ Development
Nuclei in the proliferative pseudostratified epithelia of vastly different organisms exhibit a characteristic dynamics – the so-called interkinetic nuclear migration (IKNM). Although these movements are thought to be intimately tied to the cell cycle, little is known about the relationship between IKNM and distinct phases of the cell cycle and the role that this association plays in ensuring balanced proliferation and subsequent differentiation. Here, we perform a quantitative analysis of modes of nuclear migration during the cell cycle using a marker that enables the first unequivocal differentiation of all four phases in proliferating neuroepithelial cells in vivo. In zebrafish neuroepithelia, nuclei spend the majority of the cell cycle in S phase, less time in G1, with G2 and M being noticeably shorter still in comparison. Correlating cell cycle phases with nuclear movements shows that IKNM comprises rapid apical nuclear migration during G2 phase and stochastic nuclear motion during G1 and S phases. The rapid apical migration coincides with the onset of G2, during which we find basal actomyosin accumulation. Inhibiting the transition from G2 to M phase induces a complete stalling of nuclei, indicating that IKNM and cell cycle continuation cannot be uncoupled and that progression from G2 to M is a prerequisite for rapid apical migration. Taken together, these results suggest that IKNM involves an actomyosin-driven contraction of cytoplasm basal to the nucleus during G2, and that the stochastic nuclear movements observed in other phases arise passively due to apical migration in neighboring cells.