PUBLICATION
Techniques for optimizing the creation of mutations in zebrafish using N-ethyl-N-nitrosourea
- Authors
- Trevarrow, B.
- ID
- ZDB-PUB-111027-40
- Date
- 2011
- Source
- Lab Anim. 40(11): 353-361 (Review)
- Registered Authors
- Trevarrow, Bill
- Keywords
- none
- MeSH Terms
-
- Alkylating Agents/pharmacology*
- Animals
- Animals, Genetically Modified
- Ethylnitrosourea/pharmacology*
- Genetic Techniques
- Humans
- Mutagenesis/drug effects*
- Mutagens/pharmacology*
- Mutation
- Safety/standards
- Zebrafish/genetics*
- PubMed
- 22012195 Full text @ Lab Anim.
Citation
Trevarrow, B. (2011) Techniques for optimizing the creation of mutations in zebrafish using N-ethyl-N-nitrosourea. Lab Anim.. 40(11):353-361.
Abstract
A safe and successful mutagenesis of zebrafish (Danio rerio) with N-ethyl-N-nitrosourea (ENU) involves balancing several factors. In addition to keeping the fish alive and the humans safe, labor, tank numbers and the process of finding and isolating new mutants should be considered. The author details useful techniques for optimizing zebrafish mutagenesis using ENU.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping