PUBLICATION
Characterization of testicular expression of P450 17α-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole
- Authors
- Hinfray, N., Baudiffier, D., Leal, M.C., Porcher, J.M., Aït-Aïssa, S., Le Gac, F., Schulz, R.W., and Brion, F.
- ID
- ZDB-PUB-111019-8
- Date
- 2011
- Source
- General and comparative endocrinology 174(3): 309-17 (Journal)
- Registered Authors
- Le Gac, Florence, Schulz, Rüdiger W.
- Keywords
- zebrafish, steroidogenesis, cyp17a1, pharmaceutical, clotrimazole, testis culture
- MeSH Terms
-
- Animals
- Antifungal Agents/pharmacology
- Brain/drug effects
- Brain/enzymology
- Brain/metabolism
- Cells, Cultured
- Clotrimazole/pharmacology*
- Female
- Gene Expression Regulation, Enzymologic/drug effects*
- Gonads/drug effects
- Gonads/enzymology
- Gonads/metabolism
- Male
- Primary Cell Culture
- Steroid 17-alpha-Hydroxylase/genetics*
- Steroid 17-alpha-Hydroxylase/metabolism
- Testis/chemistry
- Testis/drug effects*
- Testis/enzymology*
- Testis/metabolism
- Testosterone/analogs & derivatives
- Testosterone/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 21963961 Full text @ Gen. Comp. Endocrinol.
Citation
Hinfray, N., Baudiffier, D., Leal, M.C., Porcher, J.M., Aït-Aïssa, S., Le Gac, F., Schulz, R.W., and Brion, F. (2011) Characterization of testicular expression of P450 17α-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole. General and comparative endocrinology. 174(3):309-17.
Abstract
The aim of the present study was to characterize P450 17α-hydroxylase/17,20-lyase (cyp17a1) expression in zebrafish and to assess the effect of the pharmaceutical clotrimazole, a known inhibitor of various cytochrome P450 enzyme activities, on testicular gene and protein expression of this enzyme as well as on the testicular release of 11-ketotestosterone (11-KT), a potent androgen in fish. We first showed that cyp17a1 is predominantly expressed in gonads of zebrafish, notably in male. In vivo, clotrimazole induced a concentration-dependent increase of cyp17a1 gene expression and Cyp17-I protein synthesis in zebrafish testis. Using zebrafish testicular explants, we further showed that clotrimazole did not directly affect cyp17a1 expression but that it did inhibit 11-KT release. These novel data deserve further studies on the effect of azole fungicides on gonadal steroidogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping