PUBLICATION
Developmental lead exposure causes startle response deficits in zebrafish
- Authors
- Rice, C., Ghorai, J.K., Zalewski, K., and Weber, D.N.
- ID
- ZDB-PUB-111013-11
- Date
- 2011
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 105(3-4): 600-608 (Journal)
- Registered Authors
- Weber, Dan
- Keywords
- behavior, lead, mechanosensory, startle response, visual, zebrafish
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian
- Escape Reaction/drug effects*
- Larva/drug effects
- Larva/physiology
- Lead/pharmacokinetics
- Lead/toxicity*
- Reflex, Startle/drug effects*
- Time Factors
- Toxicity Tests, Acute
- Water Pollutants, Chemical/pharmacokinetics
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/physiology*
- PubMed
- 21955963 Full text @ Aquat. Toxicol.
Citation
Rice, C., Ghorai, J.K., Zalewski, K., and Weber, D.N. (2011) Developmental lead exposure causes startle response deficits in zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 105(3-4):600-608.
Abstract
Lead (Pb2+) exposure continues to be an important concern for fish populations. Research is required to assess the long-term behavioral effects of low-level concentrations of Pb2+ and the physiological mechanisms that control those behaviors. Newly fertilized zebrafish embryos (<2 h post fertilization; hpf) were exposed to one of three concentrations of lead (as PbCl2): 0, 10, or 30 nM until 24 hpf. (1) Response to a mechanosensory stimulus: Individual larvae (168 hpf) were tested for response to a directional, mechanical stimulus. The tap frequency was adjusted to either 1 or 4 taps/s. Startle response was recorded at 1000 fps. Larvae responded in a concentration-dependent pattern for latency to reaction, maximum turn velocity, time to reach Vmax and escape time. With increasing exposure concentrations, a larger number of larvae failed to respond to even the initial tap and, for those that did respond, ceased responding earlier than control larvae. These differences were more pronounced at a frequency of 4 taps/s. (2) Response to a visual stimulus: Fish, exposed as embryos (2–24 hpf) to Pb2+ (0–10 µM) were tested as adults under low light conditions (<60 µW/m2) for visual responses to a rotating black bar. Visual responses were significantly degraded at Pb2+ concentrations of 30 nM. These data suggest that zebrafish are viable models for short- and long-term sensorimotor deficits induced by acute, low-level developmental Pb2+ exposures.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping