ZFIN ID: ZDB-PUB-111012-5
Zebrafish models of germ cell tumor
Neumann, J.C., Lillard, K., Damoulis, V., and Amatruda, J.F.
Date: 2011
Source: Methods in cell biology   105: 1-24 (Chapter)
Registered Authors: Amatruda, James F., Damoulis, Vanessa, Lillard, Kate, Neumann, Joanie
Keywords: none
MeSH Terms:
  • Animals
  • Cell Differentiation
  • Cell Movement
  • Cell Transformation, Neoplastic/genetics
  • Cell Transformation, Neoplastic/metabolism*
  • Child
  • Child, Preschool
  • Epigenesis, Genetic
  • Flow Cytometry/methods*
  • Germ Cells/metabolism*
  • Germ Cells/pathology
  • Histones/analysis
  • Humans
  • Immunohistochemistry/methods*
  • Infant
  • Male
  • Mice
  • Neoplasms, Germ Cell and Embryonal/genetics
  • Neoplasms, Germ Cell and Embryonal/metabolism*
  • Neoplasms, Germ Cell and Embryonal/pathology
  • Primary Cell Culture
  • Spermatogenesis/genetics
  • Spermatogenesis/immunology*
  • Testicular Neoplasms/genetics
  • Testicular Neoplasms/metabolism*
  • Testicular Neoplasms/pathology
  • Testis/metabolism*
  • Testis/pathology
  • Young Adult
  • Zebrafish
PubMed: 21951524 Full text @ Meth. Cell. Biol.
Germ cell tumors are neoplasms arising from pluripotent germ cells. In humans, these tumors occur in infants, children and young adults. The tumors display a wide range of histologic differentiation states which exhibit different clinical behaviors. Information about the molecular basis of germ cell tumors, and representative animal models of these neoplasms, are lacking. Germline development in zebrafish and humans is broadly conserved, making the fish a useful model to probe the connections between germ cell development and tumorigenesis. Here, we provide an overview of germline development and a brief review of germ cell tumor biology in humans and zebrafish. We also outline some methods for studying the zebrafish germline.