PUBLICATION

Chemical screening in zebrafish for novel biological and therapeutic discovery

Authors
Tan, J.L., and Zon, L.I.
ID
ZDB-PUB-111012-25
Date
2011
Source
Methods in cell biology   105: 491-516 (Chapter)
Registered Authors
Zon, Leonard I.
Keywords
chemical screening, hit detection, pharmacodynamic, pharmacokinetic, phenotype, modulation
MeSH Terms
  • Animals
  • Automation, Laboratory
  • Behavior, Animal/drug effects
  • Behavior, Animal/physiology
  • Databases, Factual
  • Drug Discovery/methods*
  • Drug Evaluation, Preclinical/methods*
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/physiology*
  • High-Throughput Screening Assays*
  • Humans
  • Immunohistochemistry
  • Molecular Targeted Therapy/methods*
  • Phenotype
  • Research Design
  • Small Molecule Libraries/pharmacology*
  • Zebrafish/embryology
  • Zebrafish/physiology*
PubMed
21951544 Full text @ Meth. Cell. Biol.
Abstract
Zebrafish chemical screening allows for an in vivo assessment of small molecule modulation of biological processes. Compound toxicities, chemical alterations by metabolism, pharmacokinetic and pharmacodynamic properties, and modulation of cell niches can be studied with this method. Furthermore, zebrafish screening is straightforward and cost-effective. Zebrafish provide an invaluable platform for novel therapeutic discovery through chemical screening.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping