ZFIN ID: ZDB-PUB-110921-9
Genetic suppressor screens in haploids
Bai, X., Yang, Z., Jiang, H., Lin, S., and Zon, L.I.
Date: 2011
Source: Methods in cell biology   104: 129-136 (Chapter)
Registered Authors: Bai, Xiaoying, Lin, Shuo, Yang, Zhongan, Zon, Leonard I.
Keywords: suppressor screen, modifier screen, haploid, BAC transgene, ENU mutagenesis
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Chromosomes, Artificial, Bacterial/genetics
  • DNA Mutational Analysis
  • Ethylnitrosourea/pharmacology
  • Female
  • Genes, Recessive
  • Genes, Suppressor*
  • Genetic Testing/methods*
  • Haploidy
  • Larva/genetics
  • Male
  • Mutagenesis
  • Mutagens/pharmacology
  • Mutation
  • Transcription Factors/genetics
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed: 21924160 Full text @ Meth. Cell. Biol.
As a vertebrate genetic model, the zebrafish has been well recognized for its strength in studying a variety of biological processes and human diseases. Traditional forward genetic screens in zebrafish have generated a large pool of mutants with interesting phenotypes resembling human diseases but the underlying mechanisms are not well understood. A powerful approach to elucidate the mechanisms of these mutants is the modifier screen, which identifies 2(nd)-site mutations that specifically enhance or block the phenotype of a given mutant. Here we described the first genetic suppressor screen in zebrafish, which identifies a novel transcriptional mechanism regulating erythropoiesis. In combination with the haploid genetics in zebrafish, we have shown the feasibility and strength of a modifier screen in zebrafish. This strategy will greatly broaden the utility of the zebrafish as a model for making original discoveries and establishing novel paradigms for understanding vertebrate biology.