|ZFIN ID: ZDB-PUB-110921-9|
Genetic suppressor screens in haploids
Bai, X., Yang, Z., Jiang, H., Lin, S., and Zon, L.I.
|Source:||Methods in cell biology 104: 129-136 (Chapter)|
|Registered Authors:||Bai, Xiaoying, Lin, Shuo, Yang, Zhongan, Zon, Leonard I.|
|Keywords:||suppressor screen, modifier screen, haploid, BAC transgene, ENU mutagenesis|
|PubMed:||21924160 Full text @ Meth. Cell. Biol.|
Bai, X., Yang, Z., Jiang, H., Lin, S., and Zon, L.I. (2011) Genetic suppressor screens in haploids. Methods in cell biology. 104:129-136.
ABSTRACTAs a vertebrate genetic model, the zebrafish has been well recognized for its strength in studying a variety of biological processes and human diseases. Traditional forward genetic screens in zebrafish have generated a large pool of mutants with interesting phenotypes resembling human diseases but the underlying mechanisms are not well understood. A powerful approach to elucidate the mechanisms of these mutants is the modifier screen, which identifies 2(nd)-site mutations that specifically enhance or block the phenotype of a given mutant. Here we described the first genetic suppressor screen in zebrafish, which identifies a novel transcriptional mechanism regulating erythropoiesis. In combination with the haploid genetics in zebrafish, we have shown the feasibility and strength of a modifier screen in zebrafish. This strategy will greatly broaden the utility of the zebrafish as a model for making original discoveries and establishing novel paradigms for understanding vertebrate biology.
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