ZFIN ID: ZDB-PUB-110921-7
Genetic Screens for Mutations Affecting Adult Traits and Parental-effect Genes
Pelegri, F., and Mullins, M.C.
Date: 2011
Source: Methods in cell biology   104: 83-120 (Chapter)
Registered Authors: Mullins, Mary C., Pelegri, Francisco
Keywords: forward genetics, zebrafish, larval, juvenile, adult, fertility, maternal effect, paternal effect, F3-extended family screen, gynogenesis, genetic mapping
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Chromosome Mapping/methods
  • DNA Mutational Analysis/methods*
  • Ethylnitrosourea/pharmacology
  • Female
  • Genes, Recessive
  • Genetic Association Studies/methods*
  • Genetic Testing/methods*
  • Hybridization, Genetic/genetics
  • Larva/genetics
  • Larva/growth & development
  • Male
  • Morphogenesis/genetics
  • Mutagenesis/drug effects
  • Mutagens/pharmacology
  • Phenotype
  • Zebrafish/genetics*
  • Zebrafish/growth & development
PubMed: 21924158 Full text @ Meth. Cell. Biol.
Forward genetic analysis in the zebrafish has largely until now been restricted to the developmental period from the time of zygotic genome activation through the end of embryogenesis. However, the use of the zebrafish as a model system for the analysis of larval, juvenile and adult traits, including fertility and maternal and paternal effects, is gaining momentum. Here, we describe two different approaches, an F3-extended family and a gynogenesis-based approach, that allow genetic screening for and recovery of mutations affecting post-embryonic stages, including adult traits, fertility, and parental effects. For each approach, we also describe strategies to maintain and map the identified mutations.