PUBLICATION

Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon

Authors
Xi, Y., Yu, M., Godoy, R., Hatch, G., Poitras, L., and Ekker, M.
ID
ZDB-PUB-110921-43
Date
2011
Source
Developmental dynamics : an official publication of the American Association of Anatomists   240(11): 2539-47 (Journal)
Registered Authors
Ekker, Marc, Hatch, Gary, Poitras, Luc, Xi, Yanwei
Keywords
dopaminergic neuron, dopamine transporter, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Survival/genetics
  • Diencephalon/cytology
  • Diencephalon/embryology
  • Diencephalon/metabolism*
  • Dopamine Plasma Membrane Transport Proteins/genetics
  • Dopamine Plasma Membrane Transport Proteins/metabolism
  • Dopaminergic Neurons/metabolism*
  • Dopaminergic Neurons/physiology
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Gene Transfer Techniques
  • Green Fluorescent Proteins/genetics*
  • Green Fluorescent Proteins/metabolism
  • Transgenes/genetics
  • Tyrosine 3-Monooxygenase/genetics
  • Tyrosine 3-Monooxygenase/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
21932324 Full text @ Dev. Dyn.
Abstract

We have generated a line of transgenic zebrafish, Tg(dat:EGFP), in which the green fluorescent protein (GFP) is expressed under the control of cis-regulatory elements of the dopamine transporter (dat) gene. In Tg(dat:EGFP) fish, dopamine (DA) neurons are labeled with GFP, including those in ventral diencephalon (vDC) clusters, amacrine cells in the retina, in the olfactory bulb, in the pretectum, and in the caudal hypothalamus. In the vDC, DA neurons of groups 2–6 are correctly labeled with GFP, based on colocalization analyses. MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) treatments induced a modest but significant loss of DA neurons in groups 2–6 of the vDC. This transgenic line will be useful for the study of DA neuron development and in models of DA neuron loss.

Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
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Mapping