ZFIN ID: ZDB-PUB-110921-22
Sequencing-based Expression Profiling in Zebrafish
Liang, J., Renaud, G., and Burgess, S.M.
Date: 2011
Source: Methods in cell biology   104: 379-399 (Chapter)
Registered Authors: Burgess, Shawn
Keywords: amplification, annotation, cloning, microarray, profiling, tag mapping
MeSH Terms:
  • Animals
  • Base Sequence
  • Chromosome Mapping/methods
  • DNA, Complementary/biosynthesis
  • DNA, Complementary/isolation & purification
  • Expressed Sequence Tags
  • Gene Expression Profiling/methods*
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction/methods
  • RNA/genetics
  • RNA/isolation & purification
  • Sequence Analysis, DNA/methods*
  • Zebrafish/genetics*
PubMed: 21924174 Full text @ Meth. Cell. Biol.
Gene expression profiling is a powerful technique for studying biological processes, especially tissue/organ-specific ones, at the molecular level. With the rapid development of the next-generation sequencing techniques, high throughput sequencing-based expression profiling techniques have been more and more widely adopted in molecular biology studies. In this chapter, we described a protocol for applying one of the sequencing-based expression profiling techniques, Digital Gene Expression (DGE), for zebrafish research. The protocol provides guidelines for wet-bench experimental procedures as well as for bioinformatics data analyses. We also discuss potential issues/challenges with the use of DGE.