PUBLICATION
Sequencing-based Expression Profiling in Zebrafish
- Authors
- Liang, J., Renaud, G., and Burgess, S.M.
- ID
- ZDB-PUB-110921-22
- Date
- 2011
- Source
- Methods in cell biology 104: 379-399 (Chapter)
- Registered Authors
- Burgess, Shawn
- Keywords
- amplification, annotation, cloning, microarray, profiling, tag mapping
- MeSH Terms
-
- Animals
- Base Sequence
- Chromosome Mapping/methods
- DNA, Complementary/biosynthesis
- DNA, Complementary/isolation & purification
- Expressed Sequence Tags
- Gene Expression Profiling/methods*
- Molecular Sequence Data
- Oligonucleotide Array Sequence Analysis
- Polymerase Chain Reaction/methods
- RNA/genetics
- RNA/isolation & purification
- Sequence Analysis, DNA/methods*
- Zebrafish/genetics*
- PubMed
- 21924174 Full text @ Meth. Cell. Biol.
Citation
Liang, J., Renaud, G., and Burgess, S.M. (2011) Sequencing-based Expression Profiling in Zebrafish. Methods in cell biology. 104:379-399.
Abstract
Gene expression profiling is a powerful technique for studying biological processes, especially tissue/organ-specific ones, at the molecular level. With the rapid development of the next-generation sequencing techniques, high throughput sequencing-based expression profiling techniques have been more and more widely adopted in molecular biology studies. In this chapter, we described a protocol for applying one of the sequencing-based expression profiling techniques, Digital Gene Expression (DGE), for zebrafish research. The protocol provides guidelines for wet-bench experimental procedures as well as for bioinformatics data analyses. We also discuss potential issues/challenges with the use of DGE.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping