ZFIN ID: ZDB-PUB-110921-18
The Zon laboratory guide to positional cloning in zebrafish (2011)
Zhou, Y., and Zon, L.I.
Date: 2011
Source: Methods in cell biology   104: 287-309 (Chapter)
Registered Authors: Zhou, Yi, Zon, Leonard I.
Keywords: positional cloning, mutant mapping, polymorphism, SNP, allele, microsatellite marker
MeSH Terms:
  • Animal Husbandry/methods
  • Animals
  • Chromosome Mapping/methods*
  • Cloning, Molecular/methods*
  • DNA/isolation & purification
  • Female
  • Genetic Association Studies/methods
  • Genetic Linkage
  • Genetic Markers
  • Humans
  • Larva/genetics
  • Male
  • Mutation
  • Phenotype
  • Polymorphism, Single Nucleotide
  • Real-Time Polymerase Chain Reaction/methods
  • Sequence Analysis, DNA
  • Synteny
  • Takifugu/genetics
  • Zebrafish/genetics*
PubMed: 21924169 Full text @ Meth. Cell. Biol.
Zebrafish genome sequencing project has improved efficiency of positional cloning in zebrafish and traditional chromosome walking by isolating large insert genomic libraries has become a past. However, the genetic principles underlying the positional cloning still form the foundation for current chromosome walking using the genome sequence assemblies and related genomic sequence and clone information. This guide intends to summarize our accumulated experience in positional cloning using the current genomic resources and tools, and provide a practical guide to positional and/or candidate cloning of mutants of interest.