PUBLICATION
Transgenic zebrafish using transposable elements
- Authors
- Clark, K.J., Urban, M.D., Skuster, K.J., and Ekker, S.C.
- ID
- ZDB-PUB-110921-10
- Date
- 2011
- Source
- Methods in cell biology 104: 137-149 (Chapter)
- Registered Authors
- Clark, Karl, Ekker, Stephen C.
- Keywords
- zebrafish, transposon, Tol2, trangenesis, transgenic
- MeSH Terms
-
- Animals
- Animals, Genetically Modified*
- Cloning, Molecular/methods
- DNA Transposable Elements*
- Genetic Testing/methods
- Genetic Vectors
- Germ-Line Mutation
- Microinjections/methods
- Mutagenesis, Insertional/methods*
- Zebrafish/genetics*
- PubMed
- 21924161 Full text @ Meth. Cell. Biol.
Citation
Clark, K.J., Urban, M.D., Skuster, K.J., and Ekker, S.C. (2011) Transgenic zebrafish using transposable elements. Methods in cell biology. 104:137-149.
Abstract
DNA transposons are effective chromosomal engineering vehicles for making transgenic zebrafish. We describe both autonomous and non-autonomous transposable elements, and we compare and contrast popular transposon systems. The Tol2 system is a robust gene transfer tool and has been selected as the primary transposon platform, facilitating the development of an array of reagents readily shared within the zebrafish community. We present common transposon and transposase vectors within the field based on the Tol2 system. We describe methods with a high success rate of generating transgenic zebrafish using Tol2 vectors, including key quality control steps during the transgenesis process. Together, these data should enable the ready generation of transgenic zebrafish for scientific inquiry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping