Kim, S., Chung, A.Y., Kim, D., Kim, Y.S., Kim, H.S., Kwon, H.W., Huh, T.L., Park, H.C. (2011) Tcf3 function is required for the inhibition of oligodendroglial fate specification in the spinal cord of zebrafish embryos. Molecules and cells. 32(4):383-8.
The generation of various subtypes of neurons and glial cells at the right time and place is crucial for the proper development
of the vertebrate CNS. Although the mechanisms and factors for the regulation of neuronal diversity in the CNS have been well
studied, the mechanisms regulating the sequential production of neuronal and glial cells from neural precursors remain poorly
understood. This study shows that Tcf3, a member of the Lef/Tcf family of proteins, is required to inhibit the premature oligodendroglial
fate specification of spinal cord precursors using the transgenic zebrafish, which expresses a dominant repressor form of
Tcf3 under the control of a heat-shock inducible promoter. In addition, the data revealed that Tcf3 function in oligodendroglial
fate specification is mediated independently of canonical Wnt signaling. Altogether, these results show a novel function for
Tcf3 in regulating the timing of oligodendroglial fate specification in the spinal cord.