PUBLICATION
Prdm1a and miR-499 act sequentially to restrict Sox6 activity to the fast-twitch muscle lineage in the zebrafish embryo
- Authors
- Wang, X., Ono, Y., Tan, S.C., Chai, R.J., Parkin, C., and Ingham, P.W.
- ID
- ZDB-PUB-110907-10
- Date
- 2011
- Source
- Development (Cambridge, England) 138(20): 4399-404 (Journal)
- Registered Authors
- Ingham, Philip, Ono, Yosuke, Parkin, Caroline, Tan, Swee Chuan, Wang, Xingang
- Keywords
- none
- MeSH Terms
-
- Oligodeoxyribonucleotides, Antisense/genetics
- Muscle Development/genetics
- Muscle Development/physiology
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- PubMed
- 21880783 Full text @ Development
Abstract
Sox6 has been proposed to play a conserved role in vertebrate skeletal muscle fibre type specification. In zebrafish, sox6 transcription is repressed in slow-twitch progenitors by the Prdm1a transcription factor. Here we identify sox6 cis-regulatory sequences that drive fast-twitch-specific expression in a Prdm1a-dependent manner. We show that sox6 transcription subsequently becomes derepressed in slow-twitch fibres, whereas Sox6 protein remains restricted to fast-twitch fibres. We find that translational repression of sox6 is mediated by miR-499, the slow-twitch-specific expression of which is in turn controlled by Prdm1a, forming a regulatory loop that initiates and maintains the slow-twitch muscle lineage.
Genes / Markers
Figure Gallery (6 images)
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping