PUBLICATION
Feasibility study of the zebrafish assay as an alternative method to screen for developmental toxicity and embryotoxicity using a training set of 27 compounds
- Authors
- Selderslaghs, I.W., Blust, R., and Witters, H.E.
- ID
- ZDB-PUB-110901-21
- Date
- 2012
- Source
- Reproductive toxicology (Elmsford, N.Y.) 33(2): 142-154 (Journal)
- Registered Authors
- Witters, Hilda
- Keywords
- zebrafish, screening, teratogenicity, embryotoxicity, alternative method
- MeSH Terms
-
- Animal Testing Alternatives*
- Animals
- Embryo, Nonmammalian/drug effects*
- Embryonic Development/drug effects
- Feasibility Studies
- Humans
- Risk Assessment
- Teratogens/toxicity*
- Toxicity Tests/methods*
- Zebrafish*
- PubMed
- 21871558 Full text @ Reprod. Toxicol.
Citation
Selderslaghs, I.W., Blust, R., and Witters, H.E. (2012) Feasibility study of the zebrafish assay as an alternative method to screen for developmental toxicity and embryotoxicity using a training set of 27 compounds. Reproductive toxicology (Elmsford, N.Y.). 33(2):142-154.
Abstract
To anticipate to increased testing needs for reproductive toxicity and 3R approaches, we studied zebrafish embryo/larva as an alternative for animal testing for developmental toxicity and embryotoxicity and evaluated a training set of 27 compounds with a standardized protocol. The classification of compounds in the zebrafish embryo/larva assay, based on a prediction model using a TI (teratogenic index) cut-off value of 2, was compared to available animal and human data. When comparing the classification of compounds in the zebrafish embryo/larva assay to available animal classification, a sensitivity of 72% and specificity of 100% were obtained. The predictive values obtained in comparison to a limited set of human data were 50, 60% respectively for teratogens, non-teratogens.
Overall, we demonstrated that the zebrafish embryo/larva assay, may be used as screening tool for prioritization of compounds and could contribute to reduction of animal experiments in the field of developmental toxicology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping