PUBLICATION
In vivo imaging of hematopoietic stem cell development in the zebrafish
- Authors
- Zhang, P., and Liu, F.
- ID
- ZDB-PUB-110823-33
- Date
- 2011
- Source
- Frontiers of Medicine 5(3): 239-47 (Review)
- Registered Authors
- Liu, Feng, Zhang, Panpan
- Keywords
- hematooietic stem cell, hematopoiesis, in vivo imaging, transgenics, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Developmental Biology/methods
- Embryo, Nonmammalian
- Hematopoiesis/genetics
- Hematopoiesis/physiology*
- Hematopoietic Stem Cells/cytology*
- Microscopy, Confocal/methods
- Models, Genetic
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 21858412 Full text @ Front. Med.
Citation
Zhang, P., and Liu, F. (2011) In vivo imaging of hematopoietic stem cell development in the zebrafish. Frontiers of Medicine. 5(3):239-47.
Abstract
In vivo imaging is crucial for developmental biology and can further help to follow cell development/differentiation in normal and
pathological conditions. Recent advances in optical imaging techniques has facilitated tracing of the developmental dynamics
of a specific organ, tissue, or even a single cell. The zebrafish is an excellent model for imaging of hematopoiesis due to
its transparent embryo at early stage; moreover, different zebrafish hematopoietic stem cells (HSCs) transgenic lines have
been demonstrated as very useful tools for illustrating the details of the HSC developmental process. In this review, we summarize
recent studies related to the non-invasive in vivo imaging of HSC transgenics, to show that zebrafish transgenic lines are powerful tools for developmental biology and disease.
At the end of the review, the perspective and some open questions in this field will be discussed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping