PUBLICATION
Targeted gene disruption in somatic zebrafish cells using engineered TALENs
- Authors
- Sander, J.D., Cade, L., Khayter, C., Reyon, D., Peterson, R.T., Joung, J.K., and Yeh, J.R.
- ID
- ZDB-PUB-110811-21
- Date
- 2011
- Source
- Nat. Biotechnol. 29(8): 697-698 (Journal)
- Registered Authors
- Peterson, Randall, Yeh, Jing-Ruey (Joanna)
- Keywords
- none
- MeSH Terms
-
- Combinatorial Chemistry Techniques/methods*
- Genetic Engineering*
- Humans
- Mutagenesis, Site-Directed/methods*
- Transcription Factors/genetics*
- Transcription Factors/metabolism*
- PubMed
- 21822241 Full text @ Nat. Biotechnol.
Citation
Sander, J.D., Cade, L., Khayter, C., Reyon, D., Peterson, R.T., Joung, J.K., and Yeh, J.R. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat. Biotechnol.. 29(8):697-698.
Abstract
To the Editor:
Miller et al.1 recently described a transcription activator–like effector nuclease (TALEN) architecture for efficient genome editing in cultured human cells. We sought to determine whether the same framework could be used to efficiently disrupt endogenous genes in somatic cells of zebrafish and how the efficiency of TALENs compares with that obtained using engineered zinc-finger nucleases (ZFNs).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping