PUBLICATION
The mid-blastula transition defines the onset of Y RNA-dependent DNA replication in Xenopus laevis
- Authors
- Collart, C., Christov, C.P., Smith, J.C., and Krude, T.
- ID
- ZDB-PUB-110803-17
- Date
- 2011
- Source
- Molecular and cellular biology 31(18): 3857-70 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Blastula/cytology
- Blastula/physiology*
- Cell Cycle
- Cell Cycle Proteins/genetics
- Cell Cycle Proteins/metabolism
- Chromatin/metabolism
- DNA Replication*
- DNA-Binding Proteins/metabolism
- HMGA1a Protein/metabolism
- Morpholines
- Oligonucleotides, Antisense/pharmacology
- Origin Recognition Complex/metabolism
- RNA, Small Untranslated/genetics
- RNA, Small Untranslated/metabolism*
- Xenopus Proteins/metabolism
- Xenopus laevis/embryology
- Zebrafish
- PubMed
- 21791613 Full text @ Mol. Cell. Biol.
Citation
Collart, C., Christov, C.P., Smith, J.C., and Krude, T. (2011) The mid-blastula transition defines the onset of Y RNA-dependent DNA replication in Xenopus laevis. Molecular and cellular biology. 31(18):3857-70.
Abstract
Non-coding Y RNAs are essential for the initiation of chromosomal DNA replication in mammalian cell extracts, but their role in this process during early vertebrate development is unknown. Here, we use antisense morpholino nucleotides (MOs) to investigate Y RNA function in Xenopus laevis and zebrafish embryos. We show that embryos in which Y RNA function is inhibited by MOs develop normally until the mid-blastula transition (MBT) but then fail to replicate their DNA and die before gastrulation. Consistent with this observation, Y RNA function is not required for DNA replication in Xenopus egg extracts, but is required for replication in a post-MBT cell line. Y RNAs do not bind chromatin in karyomeres before MBT, but associate with interphase nuclei after MBT in an origin recognition complex (ORC)-dependent manner. Y RNA-specific MOs inhibit the association of Y RNAs with ORC, Cdt1 and HMGA1a proteins, suggesting that these molecular associations are essential for Y RNA function in DNA replication. The MBT is thus a transition point between Y RNA-independent and Y RNA-dependent control of vertebrate DNA replication. Our data suggest that in vertebrates Y RNAs function as a developmentally regulated layer of control over the evolutionarily conserved eukaryotic DNA replication machinery.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping