Deep and fast live imaging with two-photon scanned light-sheet microscopy
- Truong, T.V., Supatto, W., Koos, D.S., Choi, J.M., and Fraser, S.E.
- Nature Methods 8(9): 757-60 (Journal)
- Registered Authors
- Fraser, Scott E., Koos, David
- MeSH Terms
- Drosophila melanogaster
- Embryo, Nonmammalian/ultrastructure*
- Imaging, Three-Dimensional/instrumentation
- Imaging, Three-Dimensional/methods*
- Light/adverse effects
- 21765409 Full text @ Nat. Methods
Truong, T.V., Supatto, W., Koos, D.S., Choi, J.M., and Fraser, S.E. (2011) Deep and fast live imaging with two-photon scanned light-sheet microscopy. Nature Methods. 8(9):757-60.
We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes