PUBLICATION

Deep and fast live imaging with two-photon scanned light-sheet microscopy

Authors
Truong, T.V., Supatto, W., Koos, D.S., Choi, J.M., and Fraser, S.E.
ID
ZDB-PUB-110719-41
Date
2011
Source
Nature Methods   8(9): 757-60 (Journal)
Registered Authors
Fraser, Scott E., Koos, David
Keywords
none
MeSH Terms
  • Animals
  • Drosophila melanogaster
  • Embryo, Nonmammalian/ultrastructure*
  • Imaging, Three-Dimensional/instrumentation
  • Imaging, Three-Dimensional/methods*
  • Lasers
  • Light/adverse effects
  • Microscopy/methods*
  • Zebrafish
PubMed
21765409 Full text @ Nat. Methods
Abstract
We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes