PUBLICATION
Using intravital dyes to ubiquitously label embryonic zebrafish
- Authors
- Godinho, L.
- ID
- ZDB-PUB-110713-6
- Date
- 2011
- Source
- Cold Spring Harbor protocols 2011(7): 877-8 (Journal)
- Registered Authors
- Godinho, Leanne
- Keywords
- none
- MeSH Terms
-
- Animals
- Developmental Biology/methods*
- Fluorescent Dyes/metabolism*
- Luminescent Proteins/biosynthesis*
- Morphogenesis
- Staining and Labeling/methods*
- Zebrafish/embryology*
- PubMed
- 21724809 Full text @ Cold Spring Harb. Protoc.
Citation
Godinho, L. (2011) Using intravital dyes to ubiquitously label embryonic zebrafish. Cold Spring Harbor protocols. 2011(7):877-8.
Abstract
In the last two decades, the small tropical freshwater-dwelling zebrafish has emerged as a popular model for developmental biologists. The exceptional optical accessibility of zebrafish embryos coupled with the ability to use genetic tools to indelibly tag cells have been exploited to watch developmental events as they unfold. Moreover, because zebrafish are vertebrates, the knowledge gained through such studies is broadly applicable. This protocol describes the use of intravital dyes to ubiquitously label embryonic zebrafish. Intravital dyes such as CellTrace BODIPY TR methyl ester can be used to counterstain embryos that express fluorescent proteins either through transient injections or by stable transgenics. BODIPY TR should only be used in combination with fluorescent proteins that can be spectrally separated from it. The BODIPY TR absorption spectrum peaks at 598 nm, and its emission spectrum peaks at 625 nm. Embryos should be stained just before imaging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping