PUBLICATION
Imaging zebrafish development
- Authors
- Godinho, L.
- ID
- ZDB-PUB-110713-5
- Date
- 2011
- Source
- Cold Spring Harbor protocols 2011(7): 879-83 (Review)
- Registered Authors
- Godinho, Leanne
- Keywords
- none
- MeSH Terms
-
- Animals
- Image Processing, Computer-Assisted/methods*
- Microscopy/methods*
- Staining and Labeling/methods*
- Zebrafish/embryology*
- Zebrafish/growth & development*
- PubMed
- 21724808 Full text @ Cold Spring Harb. Protoc.
Citation
Godinho, L. (2011) Imaging zebrafish development. Cold Spring Harbor protocols. 2011(7):879-83.
Abstract
In the last two decades, the small tropical freshwater-dwelling zebrafish has emerged as a popular model for developmental biologists. The exceptional optical accessibility of zebrafish embryos coupled with the ability to use genetic tools to indelibly tag cells have been exploited to watch developmental events as they unfold. Moreover, because zebrafish are vertebrates, the knowledge gained through such studies is broadly applicable. This protocol describes the procedure for imaging zebrafish development. Once zebrafish cells have been labeled with fluorescent molecules or stained with fluorescent dye, the developmental processes of interest can be analyzed using a wide variety of live imaging techniques, such as wide-field microscopy, confocal microscopy, or multiphoton microscopy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping