PUBLICATION
Behavioral and physiological effects of acute ketamine exposure in adult zebrafish
- Authors
- Riehl, R., Kyzar, E., Allain, A., Green, J., Hook, M., Monnig, L., Rhymes, K., Roth, A., Pham, M., Razavi, R., Dileo, J., Gaikwad, S., Hart, P., and Kalueff, A.V.
- ID
- ZDB-PUB-110629-5
- Date
- 2011
- Source
- Neurotoxicology and teratology 33(6): 658-67 (Journal)
- Registered Authors
- Kalueff, Allan V.
- Keywords
- aquatic models, anxiety, locomotion and circling, cortisol, c-fos expression, behavioral testing
- MeSH Terms
-
- Animals
- Anxiety/chemically induced
- Behavior, Animal/drug effects*
- Brain/drug effects*
- Brain/metabolism
- Dose-Response Relationship, Drug
- Excitatory Amino Acid Antagonists/pharmacology*
- Excitatory Amino Acid Antagonists/toxicity
- Female
- Gene Expression/drug effects
- High-Throughput Screening Assays
- Hydrocortisone/metabolism
- Ketamine/pharmacology*
- Ketamine/toxicity
- Male
- Motor Activity/drug effects
- Polymerase Chain Reaction
- Proto-Oncogene Proteins c-fos/genetics
- Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
- Video Recording
- Zebrafish/metabolism
- Zebrafish/physiology*
- PubMed
- 21683787 Full text @ Neurotoxicol. Teratol.
Citation
Riehl, R., Kyzar, E., Allain, A., Green, J., Hook, M., Monnig, L., Rhymes, K., Roth, A., Pham, M., Razavi, R., Dileo, J., Gaikwad, S., Hart, P., and Kalueff, A.V. (2011) Behavioral and physiological effects of acute ketamine exposure in adult zebrafish. Neurotoxicology and teratology. 33(6):658-67.
Abstract
Ketamine is a non-competitive glutamatergic antagonist used to induce sedation and analgesia. In sub-anesthetic doses, it induces hyperlocomotion, impairs memory and evokes stereotypic circling in rodents. Zebrafish (Danio rerio) emerged as a promising new animal model to screen the effects of psychotropic compounds. Here, we investigated the effects of sub-anesthetic doses of ketamine on anxiety, locomotion, habituation and social behavior of adult zebrafish. Acute 20-min exposure to 20 and 40mg/L (but not 2mg/L) of ketamine reduced anxiety, impaired intra-session habituation, evoked circular swimming and disrupted zebrafish shoaling. Additionally, ketamine reduced whole-body cortisol levels and elevated brain c-fos expression in zebrafish. Our findings demonstrate the sensitivity of zebrafish to behavioral and physiological effects of sub-anesthetic doses of ketamine, further supporting the utility of this species as a model for neuropharmacological research, including testing ketamine and related drugs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping