PUBLICATION

Recording the adult zebrafish cerebral field potential during pentylenetetrazole seizures

Authors
Pineda, R., Beattie, C.E., and Hall, C.W.
ID
ZDB-PUB-110629-18
Date
2011
Source
Journal of Neuroscience Methods   200(1): 20-8 (Journal)
Registered Authors
Beattie, Christine, Pineda, Ricardo
Keywords
Epilepsy, zebrafish, EEG, pentylenetetrazole, seizure, amplifier
MeSH Terms
  • Action Potentials/drug effects
  • Action Potentials/physiology*
  • Amplifiers, Electronic*
  • Animals
  • Behavior, Animal/drug effects
  • Behavior, Animal/physiology
  • Cerebrum/drug effects
  • Cerebrum/physiopathology*
  • Convulsants/pharmacology
  • Disease Models, Animal
  • Electroencephalography/instrumentation
  • Electroencephalography/methods*
  • Electronics, Medical/instrumentation
  • Electronics, Medical/methods
  • Epilepsy/chemically induced
  • Epilepsy/physiopathology*
  • Pentylenetetrazole/pharmacology
  • Zebrafish/physiology*
PubMed
21689682 Full text @ J. Neurosci. Methods
Abstract
Although the zebrafish is increasingly used as a model organism to study epilepsy, no standard electrophysiological technique for recording electrographic seizures in adult fish exists. The purpose of this paper is to introduce a readily implementable technique for recording pentylenetetrazole seizures in the adult zebrafish. We find that we can consistently record a high quality field potential over the zebrafish cerebrum using an amplification of 5000V/V and bandpass filtering at corner frequencies of 1.6 and 16Hz. The cerebral field potential recordings show consistent features in the baseline, pre-seizure, seizure and post-seizure time periods that can be easily recognized by visual inspection as is the case with human and rodent electroencephalogram. Furthermore, numerical analysis of the field potential at the time of seizure onset reveals an increase in the total power, bandwidth and peak frequency in the power spectrum, as is also the case with human and rodent electroencephalogram. The techniques presented herein stand to advance the utility of the adult zebrafish in the study of epilepsy by affording an equivalent to the electroencephalogram used in mammalian models and human patients.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes