PUBLICATION
Single-embryo metabolomics and systematic prediction of developmental stage in zebrafish
- Authors
- Hayashi, S., Yoshida, M., Fujiwara, T., Maegawa, S., and Fukusaki, E.
- ID
- ZDB-PUB-110609-46
- Date
- 2011
- Source
- Zeitschrift fur Naturforschung. C, Journal of biosciences 66(3-4): 191-198 (Journal)
- Registered Authors
- Maegawa, Shingo
- Keywords
- Single-Embryo, Embryogenesis, Metabolomics
- MeSH Terms
-
- Animals
- Chromatography, Liquid
- Embryo, Nonmammalian/metabolism*
- Gas Chromatography-Mass Spectrometry
- Mass Spectrometry
- Metabolomics*
- Multivariate Analysis
- Zebrafish/embryology*
- PubMed
- 21630594 Full text @ Z. Naturforsch. C.
Citation
Hayashi, S., Yoshida, M., Fujiwara, T., Maegawa, S., and Fukusaki, E. (2011) Single-embryo metabolomics and systematic prediction of developmental stage in zebrafish. Zeitschrift fur Naturforschung. C, Journal of biosciences. 66(3-4):191-198.
Abstract
Metabolites, the end products of gene expression in living organisms, are tightly correlated with an organism's development and growth. Thus, metabolic profiling is a potentially important tool for understanding the events that have occurred in cells, tissues, and individual organisms. Here, we present a method for predicting the developmental stage of zebrafish embryos using novel metabolomic non-target fingerprints of "single-embryos". With this method, we observed the rate of development at different temperatures. Our results suggest that this method allows us to analyse the condition, or distinguish the genotype, of single-embryos before expression of their ultimate phenotype.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping