ZFIN ID: ZDB-PUB-110609-13
The microcephaly gene aspm is involved in brain development in zebrafish
Kim, H.T., Lee, M.S., Choi, J.H., Jung, J.Y., Ahn, D.G., Yeo, S.Y., Choi, D.K., and Kim, C.H.
Date: 2011
Source: Biochemical and Biophysical Research Communications   409(4): 640-4 (Journal)
Registered Authors: Ahn, Dae-gwon, Choi, Jung-Hwa, Kim, Cheol-Hee, Kim, Hyun-Taek, Lee, Mi-Sun, Yeo, Sang-Yeob
Keywords: Microcephaly; ASPM; CNS; Mitotic arrest; Zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Brain/embryology*
  • Cell Cycle
  • Cell Cycle Proteins/genetics*
  • Gene Knockdown Techniques
  • Humans
  • Microcephaly/genetics*
  • Mitosis/genetics
  • Molecular Sequence Data
  • Organ Size/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
PubMed: 21620798 Full text @ Biochem. Biophys. Res. Commun.
MCPH is a neurodevelopmental disorder characterized by a global reduction in cerebral cortical volume. Homozygous mutation of the MCPH5 gene, also known as ASPM, is the most common cause of the MCPH phenotype. To elucidate the roles of ASPM during embryonic development, the zebrafish aspm was identified, which is specifically expressed in proliferating cells in the CNS. Morpholino-mediated knock-down of aspm resulted in a significant reduction in head size. Furthermore, aspm-deficient embryos exhibited a mitotic arrest during early development. These findings suggest that the reduction in brain size in MCPH might be caused by lack of aspm function in the mitotic cell cycle and demonstrate that the zebrafish can provide a model system for congenital diseases of the human nervous system.