ZFIN ID: ZDB-PUB-110602-22
Nrdp1 governs differentiation of the melanocyte lineage via Erbb3b signaling in the zebrafish embryogenesis
Maddirevula, S., Anuppalle, M., Huh, T.L., Kim, S.H., and Rhee, M.
Date: 2011
Source: Biochemical and Biophysical Research Communications   409(3): 454-8 (Journal)
Registered Authors: Huh, Tae-Lin
Keywords: zebrafish, neural crest cells, nrdp1, pigmentation, erbb3b, melanocyte
MeSH Terms:
  • Animals
  • Cell Differentiation*
  • Cell Lineage*
  • Embryo, Nonmammalian/cytology
  • Gene Knockdown Techniques
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism*
  • Melanocytes/cytology*
  • Melanocytes/metabolism
  • Neural Crest/cytology
  • Neural Crest/metabolism
  • Signal Transduction
  • Transcription, Genetic
  • Ubiquitin-Protein Ligases/genetics
  • Ubiquitin-Protein Ligases/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 21596016 Full text @ Biochem. Biophys. Res. Commun.
NRDP1/FLRP1 is an E3-ubiquitin ligase with RBCC (RING, B-box, coiled-coil) motifs. NRDP1 is involved in versatile cellular signaling mechanisms in various species. Nonetheless, their functional roles in embryogenesis are largely unknown. We thus identified, isolated, and analyzed spatiotemporal expression and functional roles of zebrafish nrdp1 in the zebrafish embryogenesis. nrdp1 transcripts are prevalent in the neural crest cells, nervous system and skeletal muscle throughout the embryogenesis. Morpholino based knockdown of nrdp1 hinders pigmentation process. Based on further analysis of the nrdp1 morphants with markers for pigmentation process, we propose that Nrdp1 is associated with differentiation process of the melanocyte lineage by regulating Erbb3b, an Erbb signaling molecule along zebrafish embryogenesis.