PUBLICATION
The identification and characterization of zebrafish hematopoietic stem cells
- Authors
- Ma, D., Zhang, J., Lin, H.F., Italiano, J., and Handin, R.I.
- ID
- ZDB-PUB-110602-13
- Date
- 2011
- Source
- Blood 118(2): 289-97 (Journal)
- Registered Authors
- Handin, Robert, Lin, Hui-feng, Ma, Dongdong
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Blood Platelets/cytology
- Blood Platelets/metabolism
- Cell Differentiation/genetics
- Cell Differentiation/physiology
- Embryo, Nonmammalian
- Flow Cytometry
- Gamma Rays
- Graft Survival
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Hematopoiesis/genetics
- Hematopoiesis/physiology
- Hematopoietic Stem Cell Transplantation
- Hematopoietic Stem Cells/cytology*
- Hematopoietic Stem Cells/metabolism
- Hematopoietic Stem Cells/radiation effects
- Platelet Membrane Glycoprotein IIb/genetics
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/physiology
- PubMed
- 21586750 Full text @ Blood
Citation
Ma, D., Zhang, J., Lin, H.F., Italiano, J., and Handin, R.I. (2011) The identification and characterization of zebrafish hematopoietic stem cells. Blood. 118(2):289-97.
Abstract
Hematopoietic stem cells (HSCs) are defined by their ability to self-renew and maintain hematopoiesis throughout the lifespan
of an organism.
The optical clarity of their embryos and the ease of genetic manipulation make the zebrafish (Danio rerio) an excellent model for studying hematopoiesis. Using flow cytometry, we identified two populations of CD41-GFP+ cells (GFPhi and GFPlo) in the whole kidney marrow of Tg(CD41:GFP) zebrafish. Past studies in humans and mice have shown that CD41 is transiently
expressed in the earliest hematopoietic progenitors and is then silenced, reappearing in the platelet/thrombocyte lineage.
We have transplanted flow-sorted GFPhi and GFPlo cells into irradiated adult zebrafish and assessed long-term hematopoietic engraftment. Transplantation of GFPhi cells did not reconstitute hematopoiesis. In contrast, we observed multi-lineage hematopoiesis up to 68 weeks after primary
and secondary transplantation of GFPlo cells. We detected the CD41-GFP transgene in all major hematopoietic lineages and CD41-GFP+ cells in histologic sections of kidneys from transplant recipients. These studies show that CD41-GFPlo cells fulfill generally accepted criteria for HSCs. The identification of fluorescent zebrafish HSCs, coupled with our ability
to transplant them into irradiated adult recipients provide a valuable new tool to track HSC homing, proliferation and differentiation
into hematopoietic cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping