ZFIN ID: ZDB-PUB-110520-27
Analysis of cell proliferation, senescence, and cell death in zebrafish embryos
Verduzco, D., and Amatruda, J.F.
Date: 2011
Source: Methods in cell biology   101: 19-38 (Chapter)
Registered Authors: Amatruda, James F., Verduzco, Daniel
Keywords: cell death, cell proliferation, differentiation, dysregulation, forward-genetic screen, senescence
MeSH Terms:
  • Animals
  • Cell Death/physiology
  • Cell Growth Processes/physiology
  • Cellular Senescence/physiology
  • DNA Damage
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Humans
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed: 21550438 Full text @ Meth. Cell. Biol.
Proper control of cell proliferation is critical for normal development, growth, differentiation, and tissue homeostasis. Dysregulation of cell division and cell death underlies almost all cancers, and contributes to the pathology of birth defects and degenerative diseases. The zebrafish has proved to be an excellent system for elucidating the roles of the cell cycle in normal development, and ways in which dysregulation of cell proliferation contributes to disease. This chapter describes the methods for studying the cell cycle in zebrafish embryos, including protocols to examine cell proliferation, DNA damage, senescence, and cell death.