Endothelial Fate and Angiogenic Properties of Human CD34+ Progenitor Cells in Zebrafish
- Authors
- Pozzoli, O., Vella, P., Iaffaldano, G., Parente, V., Devanna, P., Lacovich, M., Lamia, C.L., Fascio, U., Longoni, D., Cotelli, F., Capogrossi, M.C., and Pesce, M.
- ID
- ZDB-PUB-110519-2
- Date
- 2011
- Source
- Arterioscler. Thromb. Vasc. Biol. 31(7): 1589-97 (Journal)
- Registered Authors
- Cotelli, Franco
- Keywords
- endothelium, vascular biology, angiogenesis, embryology, stem cells
- MeSH Terms
-
- Amputation, Surgical
- Animal Fins/blood supply*
- Animal Fins/surgery
- Animals
- Animals, Genetically Modified
- Antigens, CD34/analysis*
- Caco-2 Cells
- Cell Differentiation*/drug effects
- Cell Movement
- Cord Blood Stem Cell Transplantation*
- Endothelial Cells/immunology
- Endothelial Cells/transplantation*
- Fetal Blood/cytology*
- Fetal Blood/immunology
- Gene Expression Regulation, Developmental
- Hematopoietic Stem Cell Transplantation*
- Hematopoietic Stem Cells*/immunology
- Humans
- Neovascularization, Physiologic*
- Paracrine Communication
- Phenotype
- RNA Interference
- Recombinant Fusion Proteins/metabolism
- Regeneration
- Signal Transduction
- Vascular Endothelial Growth Factor C/genetics
- Vascular Endothelial Growth Factor C/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/growth & development
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 21527751 Full text @ Arterioscler. Thromb. Vasc. Biol.
Methods and Results— In the present study, human cord blood–derived CD34+ (hCD34+) cells were transplanted into pre- and postgastrulation zebrafish embryos and in an adult vascular regeneration model induced by caudal fin amputation. When injected before gastrulation, hCD34+ cells cosegregated with the presumptive zebrafish hemangioblasts, characterized by Scl and Gata2 expression, in the anterior and posterior lateral mesoderm and were involved in early development of the embryonic vasculature. These morphogenetic events occurred without apparent lineage reprogramming, as shown by CD45 expression. When transplanted postgastrulation, hCD34+ cells were recruited into developing vessels, where they exhibited a potent paracrine proangiogenic action. Finally, hCD34+ cells rescued vascular defects induced by Vegf-c in vivo targeting and enhanced vascular repair in the zebrafish fin amputation model.
Conclusion— These results indicate an unexpected developmental ability of human-derived hematopoietic progenitors and support the hypothesis of an evolutionary conservation of molecular pathways involved in endothelial progenitor differentiation in vivo.