PUBLICATION

Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation

Authors
Schnabel, K., Wu, C.C., Kurth, T., and Weidinger, G.
ID
ZDB-PUB-110519-18
Date
2011
Source
PLoS One   6(4): e18503 (Journal)
Registered Authors
Schnabel, Kristin, Weidinger, Gilbert
Keywords
none
MeSH Terms
  • Heart Diseases/physiopathology*
  • Animals
  • Regeneration*
  • Zebrafish
  • Myocardium/pathology*
  • Cold Temperature*
  • Cell Proliferation*
  • Pericardium/pathology*
(all 8)
PubMed
21533269 Full text @ PLoS One
Abstract
In mammals, myocardial cell death due to infarction results in scar formation and little regenerative response. In contrast, zebrafish have a high capacity to regenerate the heart after surgical resection of myocardial tissue. However, whether zebrafish can also regenerate lesions caused by cell death has not been tested. Here, we present a simple method for induction of necrotic lesions in the adult zebrafish heart based on cryoinjury. Despite widespread tissue death and loss of cardiomyocytes caused by these lesions, zebrafish display a robust regenerative response, which results in substantial clearing of the necrotic tissue and little scar formation. The cellular mechanisms underlying regeneration appear to be similar to those activated in response to ventricular resection. In particular, the epicardium activates a developmental gene program, proliferates and covers the lesion. Concomitantly, mature uninjured cardiomyocytes become proliferative and invade the lesion. Our injury model will be a useful tool to study the molecular mechanisms of natural heart regeneration in response to necrotic cell death.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
li1TgTransgenic Insertion
    twu34TgTransgenic Insertion
      y1TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        li1Tg
        twu34Tg
        y1Tg
        1 - 3 of 3
        Show
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        1 - 1 of 1
        Show
        Mapping
        No data available
        Your Input Welcome
        We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
        Citation
        Schnabel, K., Wu, C.C., Kurth, T., and Weidinger, G. (2011) Regeneration of cryoinjury induced necrotic heart lesions in zebrafish is associated with epicardial activation and cardiomyocyte proliferation. PLoS One. 6(4):e18503.