PUBLICATION

Ectopic expression of cone-specific G-protein-coupled receptor kinase GRK7 in zebrafish rods leads to lower photosensitivity and altered responses

Authors
Vogalis, F., Shiraki, T., Kojima, D., Wada, Y., Nishiwaki, Y., Jarvinen, J.L., Sugiyama, J., Kawakami, K., Masai, I., Kawamura, S., Fukada, Y., and Lamb,T.D.
ID
ZDB-PUB-110512-22
Date
2011
Source
The Journal of physiology   589(9): 2321-2348 (Journal)
Registered Authors
Fukada, Yoshitaka, Kawakami, Koichi, Kojima, Daisuke, Masai, Ichiro, Nishiwaki, Yuko, Shiraki, Toshiyuki
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • G-Protein-Coupled Receptor Kinase 1/genetics
  • G-Protein-Coupled Receptor Kinase 1/metabolism
  • G-Protein-Coupled Receptor Kinases/genetics
  • G-Protein-Coupled Receptor Kinases/metabolism*
  • Kinetics
  • Larva/enzymology
  • Light Signal Transduction*
  • Membrane Potentials
  • Models, Biological
  • Phosphorylation
  • Photic Stimulation
  • Retinal Rod Photoreceptor Cells/enzymology*
  • Rhodopsin/metabolism
  • Sensory Thresholds
  • Vision, Ocular
  • Zebrafish
PubMed
21486791 Full text @ J. Physiol.
Abstract
Non-technical summary When rod and cone photoreceptors in the eye respond to light, they need to recover, and the first step in recovery involves a protein called G-protein receptor kinase (GRK). Rods, which underlie night vision, employ a variant called GRK1, whereas cones, which mediate day vision, typically employ a variant called GRK7. We have engineered rod cells in the zebrafish retina that additionally express the cone variant, GRK7. By recording electrically from these modified rods, we have found that they are less sensitive to light than normal rods, in that regard mimicking cones. We have also found evidence to suggest that the size of the cell's response to a single photon (the smallest particle of light) is normal when recovery is mediated by GRK1, but is small (and hence somewhat cone-like) when mediated by GRK7. These results help us understand the differences between rod and cone photoreceptors.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes