PUBLICATION
Bioconcentration study of Xinjunan in zebrafish
- Authors
- Li, Q., Chen, S., Zhang, S., Li, C., Zhou, J., Ma, X., and Li, X.
- ID
- ZDB-PUB-110512-14
- Date
- 2011
- Source
- Environmental monitoring and assessment 183(1-4): 113-20 (Journal)
- Registered Authors
- Li, Qiang
- Keywords
- xinjunan, zebrafish, bioconcentration, acute toxicity, residual analysis, LC-MS/MS
- MeSH Terms
-
- Animals
- Chromatography, Liquid
- Polyamines/metabolism*
- Tandem Mass Spectrometry
- Zebrafish/metabolism*
- PubMed
- 21479562 Full text @ Environ. Monit. Assess.
Citation
Li, Q., Chen, S., Zhang, S., Li, C., Zhou, J., Ma, X., and Li, X. (2011) Bioconcentration study of Xinjunan in zebrafish. Environmental monitoring and assessment. 183(1-4):113-20.
Abstract
An experiment was carried out to determine the acute toxicity and bioconcentration factor of Xinjunan in zebrafish under semi-static test method. The result of the 96-h LC(50) values (0.31 mg/L), at 95% confidence limit, revealed that Xinjunan was highly toxic. Bioconcentration factor after 8 days exposure, 451.0 and 273.2, respectively, at two concentrations, were at medium bioconcentration range. To determine Xinjunan residues in water and fish, a method was developed by using a liquid-liquid distribution and a cationic exchange solid-phase extraction method to extract and clean up Xinjunan in fish, and then using a weak cationic exchange column with gradient elution and second-order mass spectrometry with selected reaction monitoring mode detection. This method found a good linear relationship (r > 0.99), the lowest limit of quantification with a signal-to-noise ratio of 10:1 was 0.02 [Formula: see text]g/L in water and 5 [Formula: see text]g/kg in fish, the recovery ranged from 97% to 109% for water and fish at different levels with a coefficient of variation less than 5%. The accuracy, precision, and lowest limit of detection of the method used for residue analysis of Xinjunan in water and fish can meet environmental exposure monitoring requirements. The results of the acute toxicity and bioconcentration provide a basis for environmental risk analysis of Xinjunan.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping