Cardona-Costa, J., Pérez-Camps, M., and García-Ximénez, F. (2012) Somatic cells derived from haploid larvae are feasible as donors for nuclear transplant in zebrafish. Preliminary results. Zygote (Cambridge, England). 20(3):277-280.
Summary Somatic cells derived from zebrafish haploid larval (both androgenetic and gynogenetic) cultures were used as donors for nuclear transplant into non-enucleated oocytes. Nuclei were transplanted either before or simultaneously with oocyte activation in the central region and in the incipient animal pole, respectively. Against expected results, 20% of transplanted embryos during oocyte activation using cells of gynogenetic origin reached the 100% epiboly stage, even two survived for up to 5 days, whereas no development was observed when cells from androgenetic origin were used. Results derived from this work open a novel possibility of studying somatic cell reprogramming and imprinting phenomena in zebrafish.