ZFIN ID: ZDB-PUB-110426-9
Somatic cells derived from haploid larvae are feasible as donors for nuclear transplant in zebrafish. Preliminary results
Cardona-Costa, J., Pérez-Camps, M., and García-Ximénez, F.
Date: 2012
Source: Zygote (Cambridge, England)   20(3): 277-280 (Journal)
Registered Authors: Cardona-Costa, Jose
Keywords: Cell culture, haploid, nuclear transplant, verebrate, zebrafish
MeSH Terms:
  • Animals
  • Cell Nucleus/metabolism
  • Cloning, Organism
  • Haploidy*
  • Larva/growth & development*
  • Nuclear Transfer Techniques
  • Oocytes/metabolism
  • Zebrafish/embryology
  • Zebrafish/metabolism*
PubMed: 21429287 Full text @ Zygote
ABSTRACT

Summary Somatic cells derived from zebrafish haploid larval (both androgenetic and gynogenetic) cultures were used as donors for nuclear transplant into non-enucleated oocytes. Nuclei were transplanted either before or simultaneously with oocyte activation in the central region and in the incipient animal pole, respectively. Against expected results, 20% of transplanted embryos during oocyte activation using cells of gynogenetic origin reached the 100% epiboly stage, even two survived for up to 5 days, whereas no development was observed when cells from androgenetic origin were used. Results derived from this work open a novel possibility of studying somatic cell reprogramming and imprinting phenomena in zebrafish.

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