Essential roles of basic helix-loop-helix transcription factors, Capsulin and Musculin, during craniofacial myogenesis of zebrafish
- Lee, G.H., Chang, M.Y., Hsu, C.H., and Chen, Y.H.
- Cellular and molecular life sciences : CMLS 68(24): 4065-78 (Journal)
- Registered Authors
- Chen, Yau-Hung
- Capsulin, Mesoderm core, Morpholino, Musculin, Myogenesis, Zebrafish
- MeSH Terms
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Basic Helix-Loop-Helix Transcription Factors/physiology*
- Cloning, Molecular
- Gene Expression Regulation, Developmental*
- Helix-Loop-Helix Motifs
- Muscle Development*
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Sequence Analysis, Protein
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Transcription Factors/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- 21347725 Full text @ Cell. Mol. Life Sci.
Lee, G.H., Chang, M.Y., Hsu, C.H., and Chen, Y.H. (2011) Essential roles of basic helix-loop-helix transcription factors, Capsulin and Musculin, during craniofacial myogenesis of zebrafish. Cellular and molecular life sciences : CMLS. 68(24):4065-78.
Capsulin and Musculin are basic helix-loop-helix transcription factors, but their biophysiological roles in zebrafish cranial myogenesis are unclear. Expressions of endogenous capsulin transcripts are detected at the central- (∼24-hpf) and at dorsal- and ventral-mesoderm cores (∼30-72 hpf) of branchial arches. In contrast, musculin transcripts are expressed as a two-phase manner: early phase (20-22 hpf) expressions of musculin are detected at the head mesoderm, whereas late-phase (36-72 hpf) are detected at all presumptive head-muscle precursors. Knockdown of either capsulin or musculin leads to loss of all cranial muscles without affecting trunk muscle development. The defective phenotypes of Capsulin- and Musculin-morphant can be rescued by co-injection of mRNA of each other. Both myf5 and myod transcripts are down-regulated in the Capsulin-morphant while myod transcripts are up-regulated in the Musculin-morphant. Therefore, we propose a putative regulatory network to understand how capsulin/musculin regulate distinctly either myf5 or myod during zebrafish craniofacial myogenesis.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes