ZFIN ID: ZDB-PUB-110316-30
Essential roles of basic helix-loop-helix transcription factors, Capsulin and Musculin, during craniofacial myogenesis of zebrafish
Lee, G.H., Chang, M.Y., Hsu, C.H., and Chen, Y.H.
Date: 2011
Source: Cellular and molecular life sciences : CMLS   68(24): 4065-78 (Journal)
Registered Authors: Chen, Yau-Hung
Keywords: Capsulin, Mesoderm core, Morpholino, Musculin, Myogenesis, Zebrafish
MeSH Terms:
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Basic Helix-Loop-Helix Transcription Factors/physiology*
  • Cloning, Molecular
  • Gene Expression Regulation, Developmental*
  • Head/embryology
  • Helix-Loop-Helix Motifs
  • Muscle Development*
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism
  • Sequence Analysis, Protein
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription Factors/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed: 21347725 Full text @ Cell. Mol. Life Sci.
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ABSTRACT
Capsulin and Musculin are basic helix-loop-helix transcription factors, but their biophysiological roles in zebrafish cranial myogenesis are unclear. Expressions of endogenous capsulin transcripts are detected at the central- (∼24-hpf) and at dorsal- and ventral-mesoderm cores (∼30-72 hpf) of branchial arches. In contrast, musculin transcripts are expressed as a two-phase manner: early phase (20-22 hpf) expressions of musculin are detected at the head mesoderm, whereas late-phase (36-72 hpf) are detected at all presumptive head-muscle precursors. Knockdown of either capsulin or musculin leads to loss of all cranial muscles without affecting trunk muscle development. The defective phenotypes of Capsulin- and Musculin-morphant can be rescued by co-injection of mRNA of each other. Both myf5 and myod transcripts are down-regulated in the Capsulin-morphant while myod transcripts are up-regulated in the Musculin-morphant. Therefore, we propose a putative regulatory network to understand how capsulin/musculin regulate distinctly either myf5 or myod during zebrafish craniofacial myogenesis.
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