PUBLICATION
Calbindin immunoreactivity in the enteric nervous system of larval and adult zebrafish (Danio rerio)
- Authors
- Olsson, C.
- ID
- ZDB-PUB-110221-20
- Date
- 2011
- Source
- Cell and tissue research 344(1): 31-40 (Journal)
- Registered Authors
- Keywords
- Enteric innervation, Immunohistochemistry, Choline acetyltransferase, Serotonin, Zebrafish, Danio rerio (Teleostei)
- MeSH Terms
-
- Animals
- Calbindins
- Enteric Nervous System/immunology*
- Larva/immunology*
- Nerve Tissue Proteins/analysis
- Nerve Tissue Proteins/immunology*
- S100 Calcium Binding Protein G/analysis
- S100 Calcium Binding Protein G/immunology*
- Zebrafish/immunology*
- PubMed
- 21327820 Full text @ Cell Tissue Res.
Citation
Olsson, C. (2011) Calbindin immunoreactivity in the enteric nervous system of larval and adult zebrafish (Danio rerio). Cell and tissue research. 344(1):31-40.
Abstract
Calbindin is a calcium-binding protein, commonly found in certain subpopulations of the enteric nervous system in mammals. Recently, calbindin-immunoreactive enteric neurons have also been demonstrated in shorthorn sculpin (Myoxocephalus scorpius). In the present study, calbindin immunoreactivity has been investigated in the gut of adult and larval zebrafish (Danio rerio) and differences and similarities between the two species are discussed. Calbindin immunoreactivity is present in 40%-50% of all enteric neurons in adult zebrafish. It first appears at 3 days post-fertilisation (dpf) and is present in all regions of the gut by 13 dpf. Calbindin-immunoreactive nerve cell bodies do not differ in size from calbindin-negative cells. Zebrafish calbindin-immunoreactive neurons are serotonin-negative, with at least some being choline acetyltransferase (ChAT)-positive, in contrast to the sculpin in which cells are generally smaller than the average enteric neuron and are serotonin-positive and ChAT-negative. These findings further emphasise the importance of comparative studies for understanding the diversity of chemical coding in the enteric nervous system of fish and other vertebrates. Improved knowledge of the role of the enteric nervous system is also essential for future studies of gut activity with regard to zebrafish being used as a model organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping