PUBLICATION
Chemical screening with zebrafish embryos
- Authors
- Zhong, H., and Lin, S.
- ID
- ZDB-PUB-110221-10
- Date
- 2011
- Source
- Methods in molecular biology (Clifton, N.J.) 716: 193-205 (Chapter)
- Registered Authors
- Lin, Shuo, Zhong, Hanbing
- Keywords
- Chemical library, Fluorescent protein, Screening, Transgenic, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Drug Evaluation, Preclinical/methods*
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Female
- Male
- Receptors, G-Protein-Coupled/antagonists & inhibitors*
- Receptors, G-Protein-Coupled/metabolism
- Small Molecule Libraries/chemistry
- Small Molecule Libraries/pharmacology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/antagonists & inhibitors*
- Zebrafish Proteins/metabolism
- PubMed
- 21318908 Full text @ Meth. Mol. Biol.
Citation
Zhong, H., and Lin, S. (2011) Chemical screening with zebrafish embryos. Methods in molecular biology (Clifton, N.J.). 716:193-205.
Abstract
Functional chemicals are very useful tools for molecular biology studies. Due to its small size, large progeny clutch, and embryonic transparency, zebrafish serves as a superb in vivo animal model for chemical compound screens and characterization. During zebrafish embryogenesis, multiple developmental phenotypes can be easily examined under the microscope, therefore allowing a more comprehensive evaluation for identifying novel functional chemicals than cell-based assays. Ever since the first zebrafish-based chemical screen was conducted in the year 2000, many functional chemicals have been discovered using this strategy. In this chapter, we describe how to perform a typical zebrafish-based chemical screen and discuss the details of the protocol by using the example of the identification and characterization of two new Smo inhibitors with a Gli:GFP transgenic line.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping