|ZFIN ID: ZDB-PUB-110221-10|
Chemical screening with zebrafish embryos
Zhong, H., and Lin, S.
|Source:||Methods in molecular biology (Clifton, N.J.) 716: 193-205 (Chapter)|
|Registered Authors:||Lin, Shuo, Zhong, Hanbing|
|Keywords:||Chemical library, Fluorescent protein, Screening, Transgenic, Zebrafish|
|PubMed:||21318908 Full text @ Meth. Mol. Biol.|
Zhong, H., and Lin, S. (2011) Chemical screening with zebrafish embryos. Methods in molecular biology (Clifton, N.J.). 716:193-205.
ABSTRACTFunctional chemicals are very useful tools for molecular biology studies. Due to its small size, large progeny clutch, and embryonic transparency, zebrafish serves as a superb in vivo animal model for chemical compound screens and characterization. During zebrafish embryogenesis, multiple developmental phenotypes can be easily examined under the microscope, therefore allowing a more comprehensive evaluation for identifying novel functional chemicals than cell-based assays. Ever since the first zebrafish-based chemical screen was conducted in the year 2000, many functional chemicals have been discovered using this strategy. In this chapter, we describe how to perform a typical zebrafish-based chemical screen and discuss the details of the protocol by using the example of the identification and characterization of two new Smo inhibitors with a Gli:GFP transgenic line.
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