ZFIN ID: ZDB-PUB-110221-10
Chemical screening with zebrafish embryos
Zhong, H., and Lin, S.
Date: 2011
Source: Methods in molecular biology (Clifton, N.J.)   716: 193-205 (Chapter)
Registered Authors: Lin, Shuo, Zhong, Hanbing
Keywords: Chemical library, Fluorescent protein, Screening, Transgenic, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Drug Evaluation, Preclinical/methods*
  • Embryo, Nonmammalian/drug effects*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Female
  • Male
  • Receptors, G-Protein-Coupled/antagonists & inhibitors*
  • Receptors, G-Protein-Coupled/metabolism
  • Small Molecule Libraries/chemistry
  • Small Molecule Libraries/pharmacology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/antagonists & inhibitors*
  • Zebrafish Proteins/metabolism
PubMed: 21318908 Full text @ Meth. Mol. Biol.
Functional chemicals are very useful tools for molecular biology studies. Due to its small size, large progeny clutch, and embryonic transparency, zebrafish serves as a superb in vivo animal model for chemical compound screens and characterization. During zebrafish embryogenesis, multiple developmental phenotypes can be easily examined under the microscope, therefore allowing a more comprehensive evaluation for identifying novel functional chemicals than cell-based assays. Ever since the first zebrafish-based chemical screen was conducted in the year 2000, many functional chemicals have been discovered using this strategy. In this chapter, we describe how to perform a typical zebrafish-based chemical screen and discuss the details of the protocol by using the example of the identification and characterization of two new Smo inhibitors with a Gli:GFP transgenic line.