PUBLICATION

Onset and dynamic expression of S100 proteins in the olfactory organ and the lateral line system in zebrafish development

Authors
Sandulescu, C.M., Teow, R., Hale, M.E., and Zhang, C.
ID
ZDB-PUB-110207-24
Date
2011
Source
Brain research   1383: 120-127 (Journal)
Registered Authors
Hale, Melina
Keywords
S100, crypt cells, neurons, olfactory system, lateral line system, developmental dynamics
MeSH Terms
  • Animals
  • Cell Differentiation/physiology
  • Immunohistochemistry
  • Larva
  • Lateral Line System/growth & development*
  • Lateral Line System/metabolism
  • Microscopy, Confocal
  • Neurogenesis/physiology*
  • Olfactory Mucosa/growth & development*
  • Olfactory Mucosa/metabolism
  • Olfactory Receptor Neurons/cytology*
  • Olfactory Receptor Neurons/metabolism
  • S100 Proteins/biosynthesis*
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
PubMed
21284940 Full text @ Brain Res.
Abstract
In the zebrafish olfactory epithelium, three morphologically distinct olfactory neurons express different marker proteins. We utilize this feature to access developmental dynamics of one of the neuron types, the crypt cells, to determine whether they are differentiated at a stage similar to other olfactory neurons. Immunohistochemical studies using an S100 antibody that specifically recognizes crypt cells showed that S100-positive cells appear in olfactory rosettes as early as at 2 day postfertilization (dpf). However, some of the rosettes did not have any S100-positive cells until 4 dpf. The number of S100-positive cells in individual rosettes increased steadily over the next 3 days before it decreased significantly. There were 7.8 S100-positive cells per rosettes on average in larvae at 7 dpf. The number reduced to 2.2 at 9 dpf. A recovery to a pre-reduction level was detected in 12 dpf larvae. We also observed S100-positive cells in neuromasts of the lateral line system in 2 dpf larvae, suggesting that the crypt cells and sensory cells in the neuromasts have similar onsets of differentiation. Our data have provided a time line of differentiation of crypt cells in development of the olfactory system and demonstrated that this type of cell is differentiated at a stage similar to ciliated and microvillous olfactory neurons. A nonlinear growth trajectory of the crypt cell population in the first nine days of zebrafish development implicates a possible functional significance of crypt cells in early life stages of zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping