PUBLICATION
Efficient mutation identification in zebrafish by microarray capturing and next generation sequencing
- Authors
- Bontems, F., Baerlocher, L., Mehenni, S., Bahechar, I., Farinelli, L., and Dosch, R.
- ID
- ZDB-PUB-110119-14
- Date
- 2011
- Source
- Biochemical and Biophysical Research Communications 405(3): 373-376 (Journal)
- Registered Authors
- Bontems, Franck, Dosch, Roland
- Keywords
- Zebrafish, Maternal effect mutation, Embryonic polarity, Embryogenesis, Genomics, Microarray capturing, Next generation sequencing, Microtubule actin crosslinking factor 1 (macf1)
- MeSH Terms
-
- Amino Acid Sequence
- Female
- Mutation
- Oligonucleotide Array Sequence Analysis/methods*
- Molecular Sequence Data
- PubMed
- 21219859 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Fish models like Medaka, stickleback or zebrafish provide a valuable resource to study vertebrate genes. However, finding genetic variants e.g. mutations in the genome is still arduous. Here we used a combination of microarray capturing and next generation sequencing to identify the affected gene in the mozartkugel(p11cv) (mzl(p11cv)) mutant zebrafish. We discovered a 31-bp deletion in macf1 demonstrating the potential of this technique to efficiently isolate mutations in a vertebrate genome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping