PUBLICATION
VIAF, a conserved inhibitor of apoptosis (IAP)-interacting factor that modulates caspase activation
- Authors
- Wilkinson, J.C., Richter, B.W., Wilkinson, A.S., Burstein, E., Rumble, J.M., Balliu, B., and Duckett, C.S.
- ID
- ZDB-PUB-110111-1
- Date
- 2004
- Source
- The Journal of biological chemistry 279(49): 51091-51099 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Motifs
- Amino Acid Sequence
- Apoptosis
- Baculoviridae/genetics
- Base Sequence
- Blotting, Northern
- Carrier Proteins/physiology*
- Caspases/metabolism*
- Cell Death
- Cell Line
- Cloning, Molecular
- Cytoplasm/metabolism
- DNA, Complementary/metabolism
- Enzyme Activation
- Glutathione Transferase/metabolism
- Green Fluorescent Proteins/metabolism
- Humans
- Inhibitor of Apoptosis Proteins
- Microscopy, Confocal
- Microscopy, Fluorescence
- Molecular Sequence Data
- Nickel/chemistry
- Open Reading Frames
- Phosphoproteins/chemistry
- Phylogeny
- Plasmids/metabolism
- Protein Structure, Tertiary
- Proto-Oncogene Proteins c-bcl-2/metabolism
- RNA Interference
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Subcellular Fractions
- Transfection
- Two-Hybrid System Techniques
- Ubiquitin/metabolism
- Viral Proteins/genetics
- bcl-2-Associated X Protein
- PubMed
- 15371430 Full text @ J. Biol. Chem.
Citation
Wilkinson, J.C., Richter, B.W., Wilkinson, A.S., Burstein, E., Rumble, J.M., Balliu, B., and Duckett, C.S. (2004) VIAF, a conserved inhibitor of apoptosis (IAP)-interacting factor that modulates caspase activation. The Journal of biological chemistry. 279(49):51091-51099.
Abstract
Inhibitor of apoptosis (IAP) proteins are involved in the suppression of apoptosis, signal transduction, cell cycle control and gene regulation. Here we describe the cloning and characterization of viral IAP-associated factor (VIAF), a highly conserved, ubiquitously expressed phosphoprotein with limited homology to members of the phosducin family that associates with baculovirus Op-IAP. VIAF bound Op-IAP both in vitro and in intact cells, with each protein displaying a predominantly cytoplasmic localization. VIAF lacks a consensus IAP binding motif, and overexpression of VIAF failed to prevent Op-IAP from protecting human cells from a variety of apoptotic stimuli, suggesting that VIAF does not function as an IAP antagonist. VIAF was unable to directly inhibit caspase activation in vitro and a reduction of VIAF protein levels by RNA interference led to a decrease in Bax-mediated caspase activation, suggesting that VIAF functions to co-regulate the apoptotic cascade. Finally, VIAF is a substrate for ubiquitination mediated by Op-IAP. Thus, VIAF is a novel IAP-interacting factor that functions in caspase activation during apoptosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping