ZFIN ID: ZDB-PUB-110110-39
APOA-II Directs Morphogenetic Movements of Zebrafish Embryo by Preventing Chromosome Fusion During Nuclear Division in Yolk Syncytial Layer
Zhang, T., Yao, S., Wang, P., Yin, C., Xiao, C., Qian, M., Liu, D., Zheng, L., Meng, W., Zhu, H., Liu, J., Xu, H., and Mo, X.
Date: 2011
Source: The Journal of biological chemistry   286(11): 9514-25 (Journal)
Registered Authors: Mo, Xianming, Wang, Ping, Yao, Shaohua, Zhang, Ting
Keywords: Apolipoproteins, Cell migration, Embryo, HDL, Zebra fish, apoa2, chromosomes fusing, division of nuclei, yolk syncytial layer
MeSH Terms:
  • Animals
  • Apolipoprotein A-II/genetics
  • Apolipoprotein A-II/metabolism*
  • Apolipoprotein A-II/pharmacology
  • Blastoderm/cytology
  • Blastoderm/metabolism*
  • Body Patterning/drug effects
  • Body Patterning/physiology*
  • Cell Nucleus Division/drug effects
  • Cell Nucleus Division/physiology*
  • Chromosomes/genetics
  • Chromosomes/metabolism
  • Giant Cells/cytology
  • Giant Cells/metabolism*
  • HeLa Cells
  • Humans
  • Morphogenesis/drug effects
  • Morphogenesis/physiology*
  • Zebrafish
PubMed: 21212265 Full text @ J. Biol. Chem.
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ABSTRACT
The high density lipoprotein (HDL) represents a class of lipid- and protein-containing particles and consists of two major apolipoproteins apoA-I and apoA-II. ApoA-II has been shown to involve in the pathogenesis of insulin resistance, adiposity, diabetes, and metabolic syndrome. In embryo, apoa2 mRNAs are abundant in the liver, brain, lung, placenta, and in fish yolk syncytial layer (YSL), suggesting that apoa2 may play function during embryonic development. Here we find out that apoa2 modulates zebrafish embryonic development by regulating the organization of YSL. Disruption of apoa2 function in zebrafish caused chromosome fusing that strongly blocked the YSL nuclear division, induced disorders in YSL organization, and finally disturbed the embryonic epiboly. Purified native human apoA-II was able specifically to rescue the defects, induced nuclear division in zebrafish embryos and in human HeLa cells. The C-terminal of apoA-II was required for the proper chromosome separation during nuclear division of YSL in zebrafish embryos and in human HeLa cells. Our data indicate that organization of YSL are required for blastoderm patterning and morphogenesis and suggest that apolipoprotein apoA-II is a novel factor of nuclear division in YSL involved in the regulation of early zebrafish embryonic morphogenesis and in the mammalian cells for proliferation.
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