PUBLICATION
Locomotor behaviors in zebrafish (Danio rerio) larvae
- Authors
- Colwill, R.M., and Creton, R.
- ID
- ZDB-PUB-101222-8
- Date
- 2011
- Source
- Behavioural processes 86(2): 222-229 (Journal)
- Registered Authors
- Creton, Robbert
- Keywords
- Activity, Danio, Larval models, Learning, Orientation, Thigmotaxis
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Female
- Image Processing, Computer-Assisted
- Larva
- Locomotion/physiology*
- Male
- Motor Activity/physiology*
- Orientation
- Video Recording
- Zebrafish/growth & development
- Zebrafish/physiology*
- PubMed
- 21147203 Full text @ Behav. Processes
Citation
Colwill, R.M., and Creton, R. (2011) Locomotor behaviors in zebrafish (Danio rerio) larvae. Behavioural processes. 86(2):222-229.
Abstract
Locomotor behaviors were examined in two experiments using zebrafish (Danio rerio) larvae at 4, 5, 6 and 7 days post fertilization (dpf). Larvae were observed in individual wells of a 12-well plate for 1h a day. In Experiment 1, the same larvae were observed for four consecutive days beginning on post-fertilization day 4; in Experiment 2, different groups of larvae from the same egg collection were observed at 4, 5, 6 and 7dpf. Automated images collected every 6s were analyzed for information about larval location, orientation and general activity. In both experiments, 4dpf larvae rested significantly more, used a smaller area of the well more frequently, and were generally less active than older larvae. All larvae exhibited a preference for facing away from the center of the well and for the edge of the well. However, prolonged exposure to the well influenced overall activity, orientation, and preference for the edge region. The implications of these results for understanding the development of larval behavior and for the design of procedures to measure the effects of experience in zebrafish larvae are discussed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping