PUBLICATION
Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish
- Authors
- Gallardo, V.E., Liang, J., Behra, M., Elkahloun, A., Villablanca, E.J., Russo, V., Allende, M.L., and Burgess, S.M.
- ID
- ZDB-PUB-101222-2
- Date
- 2010
- Source
- BMC Developmental Biology 10: 120 (Journal)
- Registered Authors
- Allende, Miguel L., Behra, Martine, Burgess, Shawn, Gallardo, Viviana
- Keywords
- none
- Datasets
- GEO:GSE25617
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Movement*
- Gene Expression Profiling
- Lateral Line System/cytology*
- Lateral Line System/embryology*
- Stem Cells/metabolism
- Zebrafish/embryology*
- PubMed
- 21144052 Full text @ BMC Dev. Biol.
Citation
Gallardo, V.E., Liang, J., Behra, M., Elkahloun, A., Villablanca, E.J., Russo, V., Allende, M.L., and Burgess, S.M. (2010) Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish. BMC Developmental Biology. 10:120.
Abstract
BACKGROUND: Development of the posterior lateral line (PLL) system in zebrafish involves cell migration, proliferation and differentiation of mechanosensory cells. The PLL forms when cranial placodal cells delaminate and become a coherent, migratory primordium that traverses the length of the fish to form this sensory system. As it migrates, the primordium deposits groups of cells called neuromasts, the specialized organs that contain the mechanosensory hair cells. Therefore the primordium provides both a model for studying collective directional cell migration and the differentiation of sensory cells from multipotent progenitor cells.
RESULTS: Through the combined use of transgenic fish, Fluorescence Activated Cell Sorting and microarray analysis we identified a repertoire of key genes expressed in the migrating primordium and in differentiated neuromasts. We validated the specific expression in the primordium of a subset of the identified sequences by quantitative RT-PCR, and by in situ hybridization. We also show that interfering with the function of two genes, f11r and cd9b, defects in primordium migration are induced. Finally, pathway construction revealed functional relationships among the genes enriched in the migrating cell population.
CONCLUSIONS: Our results demonstrate that this is a robust approach to globally analyze tissue-specific expression and we predict that many of the genes identified in this study will show critical functions in developmental events involving collective cell migration and possibly in pathological situations such as tumor metastasis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping