PUBLICATION

Chapter 2 - Imaging blood vessels in the zebrafish

Authors
Kamei, M., Isogai, S., Pan, W., and Weinstein, B.M.
ID
ZDB-PUB-101201-25
Date
2010
Source
Methods in cell biology   100: 27-54 (Chapter)
Registered Authors
Isogai, Sumio, Kamei, Makoto, Weinstein, Brant M.
Keywords
none
MeSH Terms
  • Angiography
  • Animals
  • Animals, Genetically Modified
  • Blood Vessels/cytology*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • In Situ Hybridization
  • Time-Lapse Imaging
  • Zebrafish*
PubMed
21111213 Full text @ Meth. Cell. Biol.
Abstract
Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes