ZFIN ID: ZDB-PUB-101201-25
Chapter 2 - Imaging blood vessels in the zebrafish
Kamei, M., Isogai, S., Pan, W., and Weinstein, B.M.
Date: 2010
Source: Methods in cell biology   100: 27-54 (Chapter)
Registered Authors: Isogai, Sumio, Kamei, Makoto, Weinstein, Brant M.
Keywords: none
MeSH Terms:
  • Angiography
  • Animals
  • Animals, Genetically Modified
  • Blood Vessels/cytology*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • In Situ Hybridization
  • Time-Lapse Imaging
  • Zebrafish*
PubMed: 21111213 Full text @ Meth. Cell. Biol.
Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines.