ZFIN ID: ZDB-PUB-101201-20
Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons
Tanaka, H., Nojima, Y., Shoji, W., Sato, M., Nakayama, R., Ohshima, T., and Okamoto, H.
Date: 2011
Source: Developmental dynamics : an official publication of the American Association of Anatomists   240(1): 9-22 (Journal)
Registered Authors: Nakayama, Ryoko, Okamoto, Hitoshi, Sato, Miki, Shoji, Wataru, Tanaka, Hideomi
Keywords: Islet1, Dpysl3, CRMP4, semaphorin, Rohon-Beard neuron, axon guidance, zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Axons/metabolism
  • Axons/physiology*
  • Base Sequence
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Genetic Loci
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Homeodomain Proteins/physiology*
  • LIM-Homeodomain Proteins
  • Models, Biological
  • Molecular Sequence Data
  • Motor Neurons/metabolism
  • Motor Neurons/physiology
  • Mutation/physiology
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Nerve Tissue Proteins/physiology*
  • Sensory Receptor Cells/metabolism
  • Sensory Receptor Cells/physiology*
  • Sequence Homology
  • Spinal Nerves/abnormalities
  • Spinal Nerves/embryology
  • Spinal Nerves/metabolism
  • Transcription Factors
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed: 21108318 Full text @ Dev. Dyn.
We isolated a novel zebrafish mutant, lullaby (llb), and showed that the llb locus encodes the zebrafish orthologue of isl1. Rohon-Beard (RB) primary sensory neurons are multipolar neurons that extend their central axons longitudinally within the spinal cord and also extend their peripheral axons under the skin. In llb embryos, the outgrowth of the peripheral axons of RB neurons was selectively impaired, which correlated with down-regulation of the expression of dihydropyrimidinase-like 3 (dpysl3, also known as collapsin response mediator protein 4, crmp4). Antisense morpholino oligonucleotide (AMO)-mediated knockdown of dpysl3 inhibited the outgrowth of the peripheral axons of RB neurons, and semaphorin 3d (sema3d) AMO enhanced this effect. These data indicate that Dpysl3 is cooperating with Sema3d in the peripheral axon outgrowth, and Isl1 is required for the selective outgrowth of the peripheral axons of RB neurons by maintaining the expression of dpysl3.