ZFIN ID: ZDB-PUB-101115-17
UV-induced fin damage in zebrafish as a system for evaluating the chemopreventive potential of broccoli and cauliflower extracts
Chen, Y.H., Wen, C.C., Lin, C.Y., Chou, C.Y., Yang, Z.S., and Wang, Y.H.
Date: 2011
Source: Toxicology mechanisms and methods   21(1): 63-69 (Journal)
Registered Authors: Chen, Yau-Hung
Keywords: Broccoli, cauliflower, fin, skin cancer, UVB, zebra fish
MeSH Terms:
  • Animal Fins/radiation effects*
  • Animals
  • Brassica/chemistry*
  • Larva/drug effects
  • Plant Extracts/chemistry
  • Plant Extracts/pharmacology*
  • Ultraviolet Rays*
  • Zebrafish/embryology
PubMed: 21067310 Full text @ Toxicol. Mech. Methods
ABSTRACT
This study applied broccoli and cauliflower extracts (whole, floret, and stem) to zebrafish larvae in parallel to receive 100 mJ/cm(2) of UVB six times, and recorded their fin malformation phenotypes. Chemopreventive effects of each group, including UVB, whole-, floret-, and stem-extracts of broccoli and cauliflower on fin development were evaluated using Kaplan-Meier analysis, log-rank test, and Cox proportional hazards regression. Results showed that (1) zebrafish fins in the UVB + whole broccoli extract group are 6.20~9.32-times more likely to return to normal fins than ones in the UVB only group, but fins in the UVB + whole cauliflower extract group are only 5.13~11.10-times more likely to recover, indicated that whole broccoli and cauliflower extract had similar chemopreventive ability on fin development; and (2) the broccoli stem has the highest antioxidant capacity among other groups. In conclusion, zebrafish can be used as a system for evaluating the efficacy of other UVB protective compounds.
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