PUBLICATION

Discovery of an unusual alternative splicing pathway of the immunoglobulin heavy chain in a teleost fish, Danio rerio

Authors
Hu, Y.L., Zhu, L.Y., Xiang, L.X., and Shao, J.Z.
ID
ZDB-PUB-101108-5
Date
2011
Source
Developmental and comparative immunology   35(3): 253-257 (Journal)
Registered Authors
Keywords
Zebrafish, Immunoglobulin, mIgM-2
MeSH Terms
  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Immunoglobulin Heavy Chains/chemistry
  • Immunoglobulin Heavy Chains/genetics*
  • Immunoglobulin Heavy Chains/metabolism*
  • Immunoglobulin M/chemistry
  • Immunoglobulin M/genetics
  • Immunoglobulin M/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Zebrafish/genetics*
  • Zebrafish/immunology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed
21035505 Full text @ Dev. Comp. Immunol.
Abstract
In present study, we identified a novel membrane immunoglobulin M isotype from zebrafish (Danio rerio), which was designated as mIgM-2, adding a new member to the Immunoglobulin family in teleost fish. The full length of cloned mIgM-2 cDNA was 611bp, encoding 150 amino acids. The putative mIgM-2 protein sequence consists of one constant region and a trans-membrane region. Phylogenetic analysis showed that mIgM-2 grouped with the known zebrafish IgM sequences. The mIgM-2 mRNA was widely expressed in immune-related tissues including heart, spleen, liver, intestine, kidney, gill, brain, skin and muscle. In vivo stimulation with LPS significantly up-regulates the expression of mIgM-2. Our results will add new insight into the immunoglobulin class diversity of teleost fish, and to better understand the evolutionary history of adaptive immunity from fish to mammals as a whole.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping