PUBLICATION
A new RNase sheds light on the RNase/angiogenin subfamily from zebrafish
- Authors
- Pizzo, E., Merlino, A., Turano, M., Russo Krauss, I., Coscia, F., Zanfardino, A., Varcamonti, M., Furia, A., Giancola, C., Mazzarella, L., Sica, F., and D'Alessio, G.
- ID
- ZDB-PUB-101108-33
- Date
- 2011
- Source
- The Biochemical journal 433(2): 345-355 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cells, Cultured
- Gene Expression Regulation, Developmental
- Gene Expression Regulation, Enzymologic
- Humans
- Models, Molecular
- Molecular Sequence Data
- Protein Structure, Tertiary
- Ribonuclease, Pancreatic/chemistry*
- Ribonuclease, Pancreatic/genetics
- Ribonuclease, Pancreatic/metabolism
- Ribonucleases/chemistry*
- Ribonucleases/genetics
- Ribonucleases/metabolism
- Sequence Alignment
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 21050179 Full text @ Biochem. J.
Citation
Pizzo, E., Merlino, A., Turano, M., Russo Krauss, I., Coscia, F., Zanfardino, A., Varcamonti, M., Furia, A., Giancola, C., Mazzarella, L., Sica, F., and D'Alessio, G. (2011) A new RNase sheds light on the RNase/angiogenin subfamily from zebrafish. The Biochemical journal. 433(2):345-355.
Abstract
Only recently extracellular RNases of the RNase A superfamily, with the characteristic CKxxNTF sequence signature, have been identified in fish. This has led to the recognition that these RNases are present in the whole vertebrate sub-phylum. In fact, they comprise the only enzyme family unique to vertebrates. Four RNases from zebrafish (Danio rerio) have been previously reported, with a very low RNase activity, some endowed, like human angiogenin, with powerful angiogenic and bactericidal activities. Here we report the three-dimensional structure, the thermodynamic behaviour, and the biological properties of a novel zebrafish RNase, ZF-RNase-5. The investigation of its structural and functional properties, extended to all other sub-family members, provides an inclusive description of the whole zebrafish RNase sub-family.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping