PUBLICATION
Dynamic expression patterns of 6-O endosulfatases during zebrafish development suggest a subfunctionalisation event for sulf2
- Authors
- Gorsi, B., Whelan, S., and Stringer, S.E.
- ID
- ZDB-PUB-101101-13
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(12): 3312-3323 (Journal)
- Registered Authors
- Keywords
- heparan sulfate, proteoglycans, 6-O-endosulfatases, sulf1, zebrafish, subfunctionalisation
- MeSH Terms
-
- Animals
- Central Nervous System/embryology
- Computational Biology
- Embryo, Nonmammalian/metabolism
- Heart/embryology
- Heparitin Sulfate/metabolism
- In Situ Hybridization
- Olfactory Pathways/embryology
- Proteoglycans/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 20981828 Full text @ Dev. Dyn.
Citation
Gorsi, B., Whelan, S., and Stringer, S.E. (2010) Dynamic expression patterns of 6-O endosulfatases during zebrafish development suggest a subfunctionalisation event for sulf2. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(12):3312-3323.
Abstract
The 6-O-endosulfatase enzymes (Sulfs) edit the final sulfation pattern and function of heparan sulfate (HS) by removal of 6-O-sulfate groups from the chain. To date, two mammalian sulf genes have been identified that regulate many signalling pathways during embryonic development. In zebrafish a sulf1 ortholog and duplicate copies of the mammalian sulf2 gene, sulf2a and sulf2, have been identified, which contain conserved motifs characteristic of vertebrate sulf genes. Zebrafish sulf1 and sulf2a are broadly expressed in the central nervous system (CNS) and non-neuronal tissue including heart, somite boundaries, olfactory system, and otic vesicle, whereas sulf2 expression is almost entirely restricted to the CNS. The duplicate copies of sulf2 have distinct expression patterns, which together mirror that of mouse sulf2, suggesting duplication in the teleost lineage has been followed by subfunctionalisation, whereby both genes need to be preserved by selection to ensure the ancestral gene's expression profile and function is maintained.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping