PUBLICATION

Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning

Authors
Schneider, I., Schneider, P.N., Derry, S.W., Lin, S., Barton, L.J., Westfall, T., and Slusarski, D.C.
ID
ZDB-PUB-101004-30
Date
2010
Source
Developmental Biology   348(1): 22-33 (Journal)
Registered Authors
Slusarski, Diane C.
Keywords
Naked Cuticle, Dorsal forerunner cells, Kupffer's vesicle, Left–Right patterning, Cilia, Zebrafish
MeSH Terms
  • Carrier Proteins/genetics
  • Carrier Proteins/physiology*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Molecular Sequence Data
  • Protein Stability
  • Cell Movement/drug effects
  • Oligonucleotides, Antisense/pharmacology
  • Animals
  • Adaptor Proteins, Signal Transducing/metabolism*
  • Animals, Genetically Modified
  • Phosphoproteins/metabolism*
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/isolation & purification*
  • Zebrafish Proteins/physiology*
  • beta Catenin/physiology
  • Gene Knockdown Techniques
  • Gene Expression Regulation, Developmental
  • Cilia/ultrastructure
  • Body Patterning/genetics
  • Body Patterning/physiology*
(all 24)
PubMed
20858476 Full text @ Dev. Biol.
Abstract
The establishment of the left-right (LR) axis in zebrafish embryos relies on signals from the dorsal forerunner cells (DFC) and the Kupffer's vesicle (KV). While the Wnt signaling network influences many aspects of embryonic development, its precise role in LR patterning is still unclear. One branch of the Wnt network leads to stabilization of β-catenin and activation of downstream target genes. Other Wnt ligands appear to act independently of β-catenin to modulate calcium release and influence cell polarity. Central to regulation of β-catenin and coordination of convergent extension (CE) movements is Dishevelled (Dvl). Naked Cuticle (Nkd) binds Dvl and modulates β-catenin-dependent and independent Wnt signaling. Here, we analyze the expression patterns of three zebrafish Nkd homologs and find enriched expression of nkd1 in DFCs and KV. Dvl is degraded upon Nkd1 overexpression in zebrafish. Knockdown of Nkd1 specifically in the DFC results in β-catenin nuclear localization and transcriptional activation as well as alterations to DFC migration, KV formation, ciliogenesis and LR patterning. Furthermore, we identify asymmetric expression of the Nodal antagonist charon around the KV and show that Nkd1 knockdown impacts asymmetric charon expression. Our findings show that Nkd1 acts as a β-catenin antagonist in the DFCs necessary for LR patterning.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ml1TgTransgenic Insertion
    pt6TgTransgenic Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      dnaaf11MO3-dnaaf11MRPHLNO
      ift88MO4-ift88MRPHLNO
      invsMO4-invsMRPHLNO
      nkd1MO3-nkd1MRPHLNO
      nkd1MO4-nkd1MRPHLNO
      nkd1MO5-nkd1MRPHLNO
      nkd2aMO2-nkd2aMRPHLNO
      nkd3MO1-nkd3MRPHLNO
      1 - 8 of 8
      Show
      Fish
      Fish
      ml1Tg
      pt6Tg
      pt6Tg + MO3-nkd1
      WT
      WT + MO1-nkd3
      WT + MO2-nkd2a
      WT + MO3-dnaaf11
      WT + MO3-nkd1
      WT + MO4-ift88
      WT + MO4-invs
      1 - 10 of 10
      Show
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab1-mycmonoclonal
        		IgG1Mouse
        1 - 1 of 1
        Show
        Orthology
        Gene Orthology
        nkd1
        nkd2a
        1 - 2 of 2
        Show
        Engineered Foreign Genes
        Marker Marker Type Name
        d2EGFPEFGd2EGFP
        EGFPEFGEGFP
        GFPEFGGFP
        1 - 3 of 3
        Show
        Mapping
        No data available
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        Citation
        Schneider, I., Schneider, P.N., Derry, S.W., Lin, S., Barton, L.J., Westfall, T., and Slusarski, D.C. (2010) Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning. Developmental Biology. 348(1):22-33.